CNPY3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CNPY3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15215-1-AP

Synonyms

Expanded repeat-domain protein CAG/CTG 5, ERDA5, CTG4A, CTG repeat protein 4a, canopy 3 homolog (zebrafish)

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Validation Data Gallery

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  WB analysis of human brain using 15215-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15215-1-AP

  WB result of CNPY3 antibody (15215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY3 transfected A549 cells.

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  WB analysis using 15215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15215-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 15215-1-AP

  IP result of anti-CNPY3 (IP:15215-1-AP, 3ug; Detection:15215-1-AP 1:500) with mouse kidney tissue lysate 6000ug.

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  IHC staining of human colon cancer using 15215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15215-1-AP (CNPY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15215-1-AP (CNPY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 15215-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CNPY3 antibody (15215-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, human brain tissue, A549 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

15215-1-AP targets CNPY3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNPY3 fusion protein Ag7382
• Full Name: canopy 3 homolog (zebrafish)
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC004423
• Gene Symbol: CNPY3
• Gene ID (NCBI): 10695
• RRID: AB_11182172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell Oncol (Dordr); The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells; Authors - Yao-Qi Zhou
human	WB	J Neuroimmunol; A novel rare variant of CNPY3 from familial NMOSD impairs the TLR-mediated immune response; Authors - Yongxin Mo
human	WB,IHC	World J Gastrointest Oncol; Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma; Authors - Xu-Can Gao