Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, mouse cerebellum tissue, SH-SY5Y cells, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10796-1-AP targets CNTFR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1189 Product name: Recombinant human CNTFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-372 aa of BC001492 Sequence: MAAPVPWACCAVLAAAAAVVYAQRHSPQEAPHVQYERLGSDVTLPCGTANWDAAVTWRVNGTDLAPDLLNGSQLVLHGLELGHSGLYACFHRDSWHLRHQVLLHVGLPPREPVLSCRSNTYPKGFYCSWHLPTPTYIPNTFNVTVLHGSKIMVCEKDPALKNRCHIRYMHLFSTIKYKVSISVSNALGHNATAITFDEFTIVKPDPPENVVARPVPSNPRRLEVTWQTPSTWPDPESFPLKFFLRYRPLILDQWQHVELSDGTAHTITDAYAGKEYIIQVAAKDNEIGTWSDWSVAAHATPWTEEPRHLTTEAQAAETTTSTTSSLAPPPTTKICDPGELGSGGGPSAPFLVSVPITLALAAAAATASSLLI Predict reactive species |
| Full Name | ciliary neurotrophic factor receptor |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC001492 |
| Gene Symbol | CNTFR |
| Gene ID (NCBI) | 1271 |
| RRID | AB_2083194 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26992 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ciliary neurotrophic factor (CNTF) is a pluripotent neurocytokine. The functional receptor complex of CNTF is composed of the CNTF receptor alpha (CNTFR), gp130 and the leukemia inhibitory factor receptor (LIFR) (PMID: 11943154). Binding of CNTF to CNTFR recruits gp130 and LIFR, which leads to activation of JAK/STAT and Ras/Raf/MAPK pathways.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CNTFR antibody 10796-1-AP | Download protocol |
| WB protocol for CNTFR antibody 10796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CNTFR Polyclonal antibody (Cat# 10796-1-AP) has 5 total citations. These appear in J Ethnopharmacol, Front Pharmacol, BMC Gastroenterol, Mol Biol Cell, and bioRxiv. The research fields span type 2 diabetes mellitus (insulin signaling), ischemic stroke neuroprotection, esophageal squamous cell carcinoma, and frontotemporal dementia modeling using induced pluripotent stem cells.
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Integrating metabolomics, network pharmacology and molecular dynamics simulations reveals that Xiehuang San targets CLCF1-STAT3 to restore insulin signaling in T2DM. | ||
Front Pharmacol Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier. | ||
BMC Gastroenterol MiR-21-5p regulates biological malignancy in esophageal squamous cell carcinoma via targeting CNTFR. | ||
Mol Biol Cell Patient-derived induced pluripotent stem cells with a C9orf72 expansion as a model to study frontotemporal dementia pathologies. | ||
bioRxiv Patient-derived Induced Pluripotent Stem Cells as a Model to Study Frontotemporal Dementia Pathologies |
Reviews
What customers say
Both reviews are 5-star ratings from the same user for Western Blot applications. The user reports that the antibody performs excellently, noting that the signal quality was superior compared to an alternative antibody from another company. Overall, reviewers express strong satisfaction with the product's performance and signal strength in WB experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sonia (Verified Customer) (09-15-2025) |
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Sonia (Verified Customer) (11-07-2024) | The antibody works great, we tried a different one from a different company and the signal was not as good.
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