CNTLN Polyclonal antibody

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  WB analysis using 31698-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 31698-1-AP (CNTLN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 31698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31698-1-AP (CNTLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 31698-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNTLN antibody (31698-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

CNTLN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31698-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C9orf101, C9orf39, Centlein, Centrosomal protein

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31698-1-AP targets CNTLN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36354

  Product name: Recombinant human CNTLN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 150-380 aa of

  Sequence: REKQKSEAKDRKVLEILQVKDAKIQEFEQRESVLKQEINDLVKRKIAVDEENAFLRKEFSDLEKKFKDKSQEIKDTKECVQNKEEQNRLVIKNLEEENKKLSTRCTDLLNDLEKLRKQEAHLRKEKYSTDAKIKTFEDNLIEARKEVEVSQSKYNALSLQLSNKQTELIQKDMDITLVRKELQELQNLYKQNSTHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESISY

• Full Name: centlein, centrosomal protein
• Calculated Molecular Weight: 162kDa
• Observed Molecular Weight: 162 kDa
• Gene Symbol: CNTLN
• Gene ID (NCBI): 54875
• RRID: AB_3670084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NXG0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNTLN is also named C9orf101 and C9orf39. CNTLN is required for centrosome cohesion and recruitment of CEP68 to centrosomes. CNTLN is localized at the centrosome. The calculated molecular weight of CNTLN is 162 kDa.