Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13843-1-AP targets CNTN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4803 Product name: Recombinant human CNTN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-351 aa of BC036569 Sequence: RRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNNGDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWP Predict reactive species |
| Full Name | contactin 1 |
| Calculated Molecular Weight | 1018 aa, 113 kDa |
| Observed Molecular Weight | 135 kDa |
| GenBank Accession Number | BC036569 |
| Gene Symbol | CNTN1 |
| Gene ID (NCBI) | 1272 |
| RRID | AB_2877981 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12860 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CNTN1 antibody 13843-1-AP | Download protocol |
| IP protocol for CNTN1 antibody 13843-1-AP | Download protocol |
| WB protocol for CNTN1 antibody 13843-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-CNTN1 antibody (Cat# 13843-1-AP) has accumulated 14 citations published in journals such as Nature Communications, Cell Reports Medicine, Acta Pharmacologica Sinica, JCO Precision Oncology, Journal of Biological Chemistry, and Frontiers in Immunology. Associated research fields include neuroscience (myelination, neuronal migration, diabetic cognitive dysfunction), oncology (hepatocellular, bladder, gastric, prostate, and lung cancers), ophthalmology (diabetic retinopathy), and autoimmune neurology (CIDP). Tested applications span WB, IF, IHC, and IP across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Nat Commun Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. | ||
Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.
| ||
Cell Rep Med CLDN4 palmitoylation promotes hepatic-to-biliary lineage transition and lenvatinib resistance in hepatocellular carcinoma
| ||
Acta Pharmacol Sin Clemastine alleviates cognitive dysfunction after SAH by targeting neuronal CNTN1 to regulate the ADA-adenosine pathway. | ||
Int J Surg Innovative logic "AND" gate gene circuits for bladder cancer treatment: preclinical study | ||
Front Immunol A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma |
