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  • KD/KO Validated

CNTN1 Polyclonal antibody

CNTN1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13843-1-AP

Synonyms

CNTN1, contactin 1, F3, Glycoprotein gp135, GP135, Neural cell surface protein F3



Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13843-1-AP targets CNTN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNTN1 fusion protein Ag4803
Full Name contactin 1
Calculated Molecular Weight 1018 aa, 113 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC036569
Gene Symbol CNTN1
Gene ID (NCBI) 1272
RRIDAB_2877981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).

Protocols

Product Specific Protocols
WB protocol for CNTN1 antibody 13843-1-APDownload protocol
IHC protocol for CNTN1 antibody 13843-1-APDownload protocol
IP protocol for CNTN1 antibody 13843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Authors - I-Ling Lu
  • KD Validated
ratWB

Nat Commun

Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.

Authors - Y Poitelon
humanIHC

Ann Clin Transl Neurol

CIDP/autoimmune nodopathies with nephropathy: a case series study

Authors - Yuwei Tang
mouseIF,WB

Front Mol Neurosci

Contactin-1/F3 Regulates Neuronal Migration and Morphogenesis Through Modulating RhoA Activity.

Authors - Yi-An Chen
  • KD Validated
humanWB

Front Genet

CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition.

Authors - Ruijie Zhang
humanWB

Arch Med Sci

CNTN-1 promotes docetaxel resistance and epithelial-to-mesenchymal transition via the PI3K/Akt signaling pathway in prostate cancer.

Authors - Binshen Chen