CNTN2 Recombinant monoclonal antibody

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  WB analysis using 87574-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87574-1-RR (CNTN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity of 87574-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87574-1-RR against Human CNTN2 were performed. The affinity constant is below 1 pM.

CNTN2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87574-1-RR

Clone No.252726A10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

Axonin 1, AXT, Contactin2, Contactin-2, TAG1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, mouse cerebellum tissue, pig brain tissue, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87574-1-RR targets CNTN2 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5489

  Product name: Recombinant human CNTN2 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 31-1012 aa of NM_001346083.2

  Sequence: SQTTFGPVFEDQPLSVLFPEESTEEQVLLACRARASPPATYRWKMNGTEMKLEPGSRHQLVGGNLVIMNPTKAQDAGVYQCLASNPVGTVVSREAILRFGFLQEFSKEERDPVKAHEGWGVMLPCNPPAHYPGLSYRWLLNEFPNFIPTDGRHFVSQTTGNLYIARTNASDLGNYSCLATSHMDFSTKSVFSKFAQLNLAAEDTRLFAPSIKARFPAETYALVGQQVTLECFAFGNPVPRIKWRKVDGSLSPQWTTAEPTLQIPSVSFEDEGTYECEAENSKGRDTVQGRIIVQAQPEWLKVISDTEADIGSNLRWGCAAAGKPRPTVRWLRNGEPLASQNRVEVLAGDLRFSKLSLEDSGMYQCVAENKHGTIYASAELAVQALAPDFRLNPVRRLIPAARGGEILIPCQPRAAPKAVVLWSKGTEILVNSSRVTVTPDGTLIIRNISRSDEGKYTCFAENFMGKANSTGILSVRDATKITLAPSSADINLGDNLTLQCHASHDPTMDLTFTWTLDDFPIDFDKPGGHYRRTNVKETIGDLTILNAQLRHGGKYTCMAQTVVDSASKEATVLVRGPPGPPGGVVVRDIGDTTIQLSWSRGFDNHSPIAKYTLQARTPPAGKWKQVRTNPANIEGNAETAQVLGLTPWMDYEFRVIASNILGTGEPSGPSSKIRTREAAPSVAPSGLSGGGGAPGELIVNWTPMSREYQNGDGFGYLLSFRRQGSTHWQTARVPGADAQYFVYSNESVRPYTPFEVKIRSYNRRGDGPESLTALVYSAEEEPRVAPTKVWAKGVSSSEMNVTWEPVQQDMNGILLGYEIRYWKAGDKEAAADRVRTAGLDTSARVSGLHPNTKYHVTVRAYNRAGTGPASPSANATTMKPPPRRPPGNISWTFSSSSLSIKWDPVVPFRNESAVTGYKMLYQNDLHLTPTLHLTGKNWIEIPVPEDIGHALVQIRTTGPGGDGIPAEVHIVRNGGTSMMVEN

• Full Name: contactin 2 (axonal)
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 135~150 kDa
• GenBank Accession Number: NM_001346083.2
• Gene Symbol: CNTN2
• Gene ID (NCBI): 6900
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02246
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Contactin-2 (CNTN2), also known as CNTN2 or axonal glycoprotein TAG-1, is a cell adhesion molecule anchored within the cell membrane that is a member of the immunoglobulin family. It is reported to be transiently expressed in specific neurons during the fetal period (PMID: 27621318, 29777776). CNTN2 consists of six Ig domains followed by four fibronectin domains, and it is tethered to the cell surface by a glycosylphosphatidylinositol anchor. CNTN2 levels may reflect impairment of brain development and indirectly brain iron stores, which are likely deficient in infants of maternal IDA or chronic hypoxic states like IDMs (PMID: 26718491).