CNTN4 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• CNTN4 Recombinant monoclonal antibody (87572-1-RR)

• 

  WB analysis of fetal human brain using 87572-1-RR

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 87572-1-RR (CNTN4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87572-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87572-1-RR against Human CNTN4 were performed. The affinity constant is below 1 pM.

CNTN4 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87572-1-RR

Clone No.252031B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Contactin-4, AXCAM, BIG 2, BIG-2, Brain-derived immunoglobulin superfamily protein 2

---

Tested Applications

• Positive WB detected in: fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87572-1-RR targets CNTN4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5574

  Product name: Recombinant human CNTN4/Contactin-4 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 19-1000 aa of NM_001206955.2

  Sequence: DDSTLHGPIFIQEPSPVMFPLDSEEKKVKLNCEVKGNPKPHIRWKLNGTDVDTGMDFRYSVVEGSLLINNPNKTQDAGTYQCTATNSFGTIVSREAKLQFAYLDNFKTRTRSTVSVRRGQGMVLLCGPPPHSGELSYAWIFNEYPSYQDNRRFVSQETGNLYIAKVEKSDVGNYTCVVTNTVTNHKVLGPPTPLILRNDGVMGEYEPKIEVQFPETVPTAKGATVKLECFALGNPVPTIIWRRADGKPIARKARRHKSNGILEIPNFQQEDAGLYECVAENSRGKNVARGQLTFYAQPNWIQKINDIHVAMEENVFWECKANGRPKPTYKWLKNGEPLLTRDRIQIEQGTLNITIVNLSDAGMYQCLAENKHGVIFSNAELSVIAVGPDFSRTLLKRVTLVKVGGEVVIECKPKASPKPVYTWKKGRDILKENERITISEDGNLRIINVTKSDAGSYTCIATNHFGTASSTGNLVVKDPTRVMVPPSSMDVTVGESIVLPCQVTHDHSLDIVFTWSFNGHLIDFDRDGDHFERVGGQDSAGDLMIRNIQLKHAGKYVCMVQTSVDRLSAAADLIVRGPPGPPEAVTIDEITDTTAQLSWRPGPDNHSPITMYVIQARTPFSVGWQAVSTVPELIDGKTFTATVVGLNPWVEYEFRTVAANVIGIGEPSRPSEKRRTEEALPEVTPANVSGGGGSKSELVITWETVPEELQNGRGFGYVVAFRPYGKMIWMLTVLASADASRYVFRNESVHPFSPFEVKVGVFNNKGEGPFSPTTVVYSAEEEPTKPPASIFARSLSATDIEVFWASPLEKNRGRIQGYEVKYWRHEDKEENARKIRTVGNQTSTKITNLKGSVLYHLAVKAYNSAGTGPSSATVNVTTRKPPPSQPPGNIIWNSSDSKIILNWDQVKALDNESEVKGYKVLYRWNRQSSTSVIETNKTSVELSLPFDEDYIIEIKPFSDGGDGSSSEQIRIPKISNAYARGS

• Full Name: contactin 4
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_001206955.2
• Gene Symbol: CNTN4
• Gene ID (NCBI): 152330
• RRID: AB_3745626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IWV2-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Contactins are nervous system-specific members of the immunoglobulin superfamily, comprising six subtypes: contactin, TAG-1, BIG-1, BIG-2, NB-2 and NB-3 (PMID: 19262165). They are structurally and functionally conserved among species and predominantly expressed in neurons (PMID: 25300137). As a GPI-anchored synaptic adhesion molecule, CNTN4 (BIG-2) regulates brain axonal activities (PMID: 14571131). Elevated CNTN4 expression in tumors suppresses the anti-tumor immunity of T cells (PMID: 39392894).