Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12991-1-AP targets COASY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3651 Product name: Recombinant human COASY protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 296-564 aa of BC020985 Sequence: MAINRFRLENDLEELALYQIQLLKDLRHTENEEDKVSSSSFRQRMLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD Predict reactive species |
| Full Name | Coenzyme A synthase |
| Calculated Molecular Weight | 564 aa, 62 kDa |
| Observed Molecular Weight | 62 kDa |
| GenBank Accession Number | BC020985 |
| Gene Symbol | COASY |
| Gene ID (NCBI) | 80347 |
| RRID | AB_2083528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13057 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the fourth and fifth sequential steps of the CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. It was reported that having a key role in erythroid maturation and identified upstream and downstream metabolites of COASY as a potential treatment for anemia in patients with MDS-RS (PMID: 36857430). COASY has 2 isoforms with molecular weights of 62 and 65 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COASY antibody 12991-1-AP | Download protocol |
| IHC protocol for COASY antibody 12991-1-AP | Download protocol |
| IP protocol for COASY antibody 12991-1-AP | Download protocol |
| WB protocol for COASY antibody 12991-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The COASY Polyclonal antibody (Cat# 12991-1-AP) has 2 total citations. These publications appear in Frontiers in Pharmacology and Molecular Nutrition & Food Research. The cited research spans metabolomics and network pharmacology (investigating Xin-Sheng-Hua Granule for anemia treatment) and purine biosynthesis/metabolic pathway studies (examining fructose overconsumption effects in rats). Both studies utilized the antibody for Western blot, with one also employing immunofluorescence, in rat models.
| Species | Application | Title |
|---|---|---|
Front Pharmacol Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia. | ||
Mol Nutr Food Res Prolonged Fructose Overconsumption Activates Original Biosynthetic and Metabolic Pathways of Endogenous Purine in Rats |
Reviews
What customers say
The single review for this product (Western Blot, dilution 1/1000) reports that expression was detected in Hela cells and hepatic tissue, but not in fibroblasts. The reviewer rated it 3 out of 5, reflecting partial success across the tested cell types and tissues. Overall, the antibody performed as expected in positive samples but failed to detect expression in fibroblasts, which may indicate limited reactivity or low/absent target expression in that cell line.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Durand (Verified Customer) (07-22-2026) | Expression detected in Hela cells, but not in Fibroblasts.
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