COASY Polyclonal antibody

COASY Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12991-1-AP

Synonyms

DPCK, Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase

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Validation Data Gallery

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  WB analysis of HeLa using 12991-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12991-1-AP (COASY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12991-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12991-1-AP (COASY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12991-1-AP

  IP result of anti-COASY (IP:12991-1-AP, 4ug; Detection:12991-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human colon cancer using 12991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12991-1-AP (COASY antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 12991-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12991-1-AP (COASY antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12991-1-AP targets COASY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COASY fusion protein Ag3651
• Full Name: Coenzyme A synthase
• Calculated Molecular Weight: 564 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC020985
• Gene Symbol: COASY
• Gene ID (NCBI): 80347
• RRID: AB_2083528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the fourth and fifth sequential steps of the CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. It was reported that having a key role in erythroid maturation and identified upstream and downstream metabolites of COASY as a potential treatment for anemia in patients with MDS-RS (PMID: 36857430). COASY has 2 isoforms with molecular weights of 62 and 65 kDa.

Publications

Species	Application	Title
rat	WB	Front Pharmacol; Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.; Authors - Han-Qing Pang