COG1 Polyclonal antibody

COG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10767-1-AP

Synonyms

CDG2G, COG complex subunit 1, COG1, KIAA1381, LDLB

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Validation Data Gallery

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  WB analysis using 10767-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10767-1-AP (COG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10767-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10767-1-AP (COG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10767-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10767-1-AP (COG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10767-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10767-1-AP (COG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U-251 cells, PC-3 cells, U2OS cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10767-1-AP targets COG1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COG1 fusion protein Ag1203
• Full Name: component of oligomeric golgi complex 1
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 109 kDa
• GenBank Accession Number: BC021985
• Gene Symbol: COG1
• Gene ID (NCBI): 9382
• RRID: AB_2083677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells (PMID: 16537452). It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa.