Collagen Type XIII Polyclonal antibody

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  WB analysis using 19801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19801-1-AP (Collagen Type XIII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Collagen Type XIII Polyclonal Antibody for WB, ELISA

Cat No. 19801-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

COL13, COL13A1, Collagen alpha 1(XIII) chain, Collagen alpha-1(XIII) chain, COLXIIIA1

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Tested Applications

• Positive WB detected in: HT-1080 cells, NIH/3T3 eclls

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19801-1-AP targets Collagen Type XIII in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: collagen, type XIII, alpha 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 62-70 kDa, 85-100 kDa
• GenBank Accession Number: NM_001130103
• Gene Symbol: COL13A1
• Gene ID (NCBI): 1305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5TAT6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL13A1 is involved in cell-matrix and cell-cell adhesion interactions that are required for normal development. COL13A1 may participate in the linkage between muscle fiber and basement membrane. COL13A1 may play a role in endochondral ossification of bone and branching morphogenesis of lung. COL13A1 can bind heparin. The antibody can recognize all the isoforms except isoform9 of COL13A1. This antibody is specific to COL13A1. It is predicted molecular weight is 62-70 kDa, and it is also reported as 85-95 kDa (PMID:11223332).