COL4A4 Polyclonal antibody

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• COL4A4 Polyclonal antibody (19674-1-AP)

COL4A4 Polyclonal Antibody for IF/ICC, ELISA

Cat No. 19674-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

COL4, Collagen Type IV, COL4A 4, collagen, type IV, alpha 4

• 

  IF Staining of HeLa using 19674-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19674-1-AP (Collagen Type IV antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19674-1-AP targets COL4A4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: collagen, type IV, alpha 4
• Calculated Molecular Weight: 164 kDa
• GenBank Accession Number: NM_000092
• Gene Symbol: COL4A4
• Gene ID (NCBI): 1286
• RRID: AB_2878595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53420
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL4A4 belongs to the type IV collagen family. COL4A4 is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A4 are a cause of Alport syndrome autosomal recessive (APSAR). Defects in COL4A4 are a cause of benign familial hematuria (BFH). This antibody is specific to COL4A4.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell; Early rhombic lip Protogenin+ve stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma; Authors - Abhirami Visvanathan
human	IF	Bioact Mater; Bioengineering an improved three-dimensional vascularized co-culture model for studying Neuron-Microglia interactions.; Authors - Yinhe Han
mouse	IF	Cell Stem Cell; Enhanced viability and functional maturity of iPSC-derived islet organoids by collagen-VI-enriched ECM scaffolds; Authors - Deliang Zhu
mouse	IHC	Cell Rep Med; Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity; Authors - Li Wang
mouse	IF	Biomed Pharmacother; Glucosamine alleviates liver fibrosis through modulation of sinusoidal blood flow and hepatic immune responses; Authors - Xin Chen
		Genomics Proteomics Bioinformatics; Spatial Transcriptomics Unveils the Blueprint of Mammalian Lung Development.; Authors - Mingyue Chen