Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, HepG2 cells, COLO 320 cells, mouse kidney tissue, mouse spleen tissue, rat kidney tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17251-1-AP targets COL8A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10607 Product name: Recombinant human COL8A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 396-744 aa of BC013581 Sequence: GIPGPMGPPGAIGFPGPKGEGGIVGPQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEPGIPGDQGLQGPPGIPGIGGPSGPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGPQGQPGLPGPPGPPGPPGPPAVMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAFTAELTAPFPPVGAPVKFNKLLYNGRQNYNPQTGIFTCEVPGVYYFAYHVHCKGGNVWVALFKNNEPVMYTYDEYKKGFLDQASGSAVLLLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGYLLYPM Predict reactive species |
| Full Name | collagen, type VIII, alpha 1 |
| Calculated Molecular Weight | 744 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC013581 |
| Gene Symbol | COL8A1 |
| Gene ID (NCBI) | 1295 |
| RRID | AB_10696173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27658 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COL8A1 antibody 17251-1-AP | Download protocol |
| IP protocol for COL8A1 antibody 17251-1-AP | Download protocol |
| WB protocol for COL8A1 antibody 17251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-COL8A1 antibody (Cat# 17251-1-AP) has 16 citations spanning journals including Sci China Life Sci, Am J Hum Genet, FASEB J, iScience, J Neurochem, Cell Rep Methods, Front Cell Dev Biol, Exp Cell Res, and others. Research fields cover ophthalmology (myopia, cornea), cardiac fibrosis and cardiotoxicity, glioma and thyroid cancer prognosis, extracellular matrix biology, EMT, aortopathy, and developmental disorders such as Schuurs-Hoeijmakers syndrome and esophageal atresia.
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Allele-specific RNAi therapy corrects an extracellular matrix defect in Schuurs-Hoeijmakers syndrome.
| ||
Front Bioeng Biotechnol Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts. | ||
Cell Rep Methods Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugs. | ||
Invest Ophthalmol Vis Sci Comparative Transcriptomic Profiling of Corneal Compartments Using Single-Cell and Single-Nucleus Sequencing. | ||
Front Cell Dev Biol Platelet-Derived Extracellular Vesicles Increase Col8a1 Secretion and Vascular Stiffness in Intimal Injury.
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Reviews
What customers say
The single review reports a 5-star rating for this antibody used in immunofluorescence on mouse eye cryosections at a 1:100 dilution. The user noted that the antibody worked well for the application. Overall, the feedback is positive, confirming reliable performance in IF on mouse tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kenzo (Verified Customer) (11-11-2023) | This antibody worked well for mouse eye cryosections.
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