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COMMD1 Polyclonal antibody
COMMD1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 11938-1-AP
|Positive WB detected in||human liver tissue, HeLa cells, HepG2 cells, human brain tissue, human heart tissue, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11938-1-AP targets COMMD1 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COMMD1 fusion protein Ag2535|
|Full Name||copper metabolism (Murr1) domain containing 1|
|Calculated molecular weight||190 aa, 21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC022046|
|Gene ID (NCBI)||150684|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.
|Product Specific Protocols|
|WB protocol for COMMD1 antibody 11938-1-AP||Download protocol|
|IHC protocol for COMMD1 antibody 11938-1-AP||Download protocol|
|IF protocol for COMMD1 antibody 11938-1-AP||Download protocol|
|IP protocol for COMMD1 antibody 11938-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta
A cell-type-specific role for murine Commd1 in liver inflammation.
Biol Trace Elem Res
The Alteration of Copper Homeostasis in Inflammation Induced by Lipopolysaccharides.
COMMD1-mediated ubiquitination regulates CFTR trafficking.
Int J Biochem Cell Biol
COMMD1 modulates noxious inflammation in cystic fibrosis.
Roles of COMM-domain-containing 1 in stability and recruitment of the copper-transporting ATPase in a mouse hepatoma cell line.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emilia (Verified Customer) (11-30-2018)
works well for WB