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COMMD1 Polyclonal antibody
COMMD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 11938-1-AP
|Positive WB detected in||HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11938-1-AP targets COMMD1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COMMD1 fusion protein Ag2535|
|Full Name||copper metabolism (Murr1) domain containing 1|
|Calculated molecular weight||190 aa, 21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC022046|
|Gene ID (NCBI)||150684|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.
|Product Specific Protocols|
|WB protocol for COMMD1 antibody 11938-1-AP||Download protocol|
|IHC protocol for COMMD1 antibody 11938-1-AP||Download protocol|
|IF protocol for COMMD1 antibody 11938-1-AP||Download protocol|
|IP protocol for COMMD1 antibody 11938-1-AP||Download protocol|
|Click here to view our Standard Protocols|
COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.
A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.
CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.
J Cell Biol
HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.
COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.
SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emilia (Verified Customer) (11-30-2018)
works well for WB