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  • KD/KO Validated

COMMD1 Polyclonal antibody

COMMD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11938-1-AP

Synonyms

C2orf5, COMMD1, MURR1, Protein Murr1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11938-1-AP targets COMMD1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COMMD1 fusion protein Ag2535
Full Name copper metabolism (Murr1) domain containing 1
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC022046
Gene symbol COMMD1
Gene ID (NCBI) 150684
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

Protocols

Product Specific Protocols
WB protocol for COMMD1 antibody 11938-1-APDownload protocol
IHC protocol for COMMD1 antibody 11938-1-APDownload protocol
IF protocol for COMMD1 antibody 11938-1-APDownload protocol
IP protocol for COMMD1 antibody 11938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Circ Res

COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.

Authors - Alina Fedoseienko
  • KD Validated
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
canineWB,IF

Nat Commun

CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.

Authors - Paulina Bartuzi
  • KO Validated
human

J Cell Biol

HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.

Authors - Ewan MacDonald
humanWB,IF,CoIP

Cell Rep

COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.

Authors - Carole G Campion
  • KD Validated
humanWB

Theranostics

SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.

Authors - Ling Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emilia (Verified Customer) (11-30-2018)

works well for WB

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1;1000
  • Cell Tissue Type: 293, Cos1