Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11938-1-AP targets COMMD1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2535 Product name: Recombinant human COMMD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC022046 Sequence: MAAGELEGGKPLSGLLNALAQDTFHGYPGITEELLRSQLYPEVPPEEFRPFLAKMRGILKSIASADMDFNQLEAFLTAQTKKQGGITSDQAAVISKFWKSHKTKIRESLMNQSRWNSGLRGLSWRVDGKSQSRHSAQIHTPVAIIELELGKYGQESEFLCLEFDEVKVNQILKTLSEVEESISTLISQPN Predict reactive species |
| Full Name | copper metabolism (Murr1) domain containing 1 |
| Calculated Molecular Weight | 190 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC022046 |
| Gene Symbol | COMMD1 |
| Gene ID (NCBI) | 150684 |
| RRID | AB_2083542 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N668 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COMMD1 antibody 11938-1-AP | Download protocol |
| IHC protocol for COMMD1 antibody 11938-1-AP | Download protocol |
| IP protocol for COMMD1 antibody 11938-1-AP | Download protocol |
| WB protocol for COMMD1 antibody 11938-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The COMMD1 Polyclonal antibody (Cat# 11938-1-AP) has 30 citations published in journals including Cell, Nature Communications, Circulation Research, Science Immunology, Cell Death & Differentiation, Oncogene, Cell Reports, EMBO Reports, Journal of Cell Biology, and PLoS One. Research fields encompass endosomal trafficking, LDL/cholesterol metabolism, copper homeostasis, cystic fibrosis, cancer (hepatocellular, ovarian, cervical), inflammation, immune regulation, neurodegeneration, atherosclerosis, cuproptosis, and fibrosis.
| Species | Application | Title |
|---|---|---|
Nat Commun CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.
| ||
Nat Commun A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. | ||
Circ Res COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.
| ||
Sci Immunol Celastrol suppresses humoral immune responses and autoimmunity by targeting the COMMD3/8 complex | ||
Theranostics SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The user reported that the antibody "works well for WB" (Western Blot) when used on 293 and Cos1 cells at a 1:1000 dilution. Overall, the feedback indicates reliable performance for Western Blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emilia (Verified Customer) (11-30-2018) | works well for WB
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