COMMD1 Monoclonal antibody

COMMD1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3B10E10

Cat no : 67016-1-Ig

Synonyms

C2orf5, COMMD1, MURR1, Protein Murr1



Tested Applications

Positive WB detected inHT-29 cells, A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67016-1-Ig targets COMMD1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COMMD1 fusion protein Ag28596
Full Name copper metabolism (Murr1) domain containing 1
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC022046
Gene symbol COMMD1
Gene ID (NCBI) 150684
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

Protocols

Product Specific Protocols
WB protocol for COMMD1 antibody 67016-1-IgDownload protocol
IHC protocol for COMMD1 antibody 67016-1-IgDownload protocol
IF protocol for COMMD1 antibody 67016-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols