COPB1 Polyclonal antibody

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• COPB1 Polyclonal antibody (27469-1-AP)

• KD/KO Validated

COPB1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27469-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Beta coat protein, Beta COP, Beta-coat protein, Beta-COP, Coatomer subunit beta

• 

  WB analysis using 27469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27469-1-AP (COPB1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 27469-1-AP

  WB result of COPB1 antibody (27469-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COPB1 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 27469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27469-1-AP (COPB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 27469-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27469-1-AP (COPB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27469-1-AP targets COPB1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26518

  Product name: Recombinant human COPB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 288-544 aa of BC037280

  Sequence: LIIKESDNNVKLIVLDRLIELKEHPAHERVLQDLVMDILRVLSTPDLEVRKKTLQLALDLVSSRNVEELVIVLKKEVIKTNNVSEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDNNEAAAADVLEFVREAIQRFDNLRMLIVEKMLEVFHAIKSVKIYRGALWILGEYCSTKEDIQSVMTEIRRSLGEIPIVESEIKKEAGELKPEEEITVGPVQKLVTEMGTYATQSALSSSRPTKKEEDRPPLRG

• Full Name: coatomer protein complex, subunit beta 1
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC037280
• Gene Symbol: COPB1
• Gene ID (NCBI): 1315
• RRID: AB_2880880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53618
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The COPB1 Polyclonal antibody (Cat# 27469-1-AP) has 3 total citations. These appear in Science Advances and bioRxiv (two preprints). The cited research spans cell senescence (COPI protein roles), peroxisome degradation and ischemia protection (ether glycerophospholipid signaling), and skeletal muscle mass maintenance linked to nutrient and oxygen availability (DEPP1 pathway). The antibody was used in immunoprecipitation (IP) applications in human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IP	Sci Adv; Distinct roles of COPI proteins attenuated in cell senescence.; Authors - Krystyna Mazan-Mamczarz
		bioRxiv; DEPP1 connects nutrient and oxygen availability to maintenance of muscle mass.; Authors - Shariq Qayyum
		bioRxiv; Ether glycerophospholipid control of peroxisome degradation confers protection from ischemia.; Authors - Madhu Singh