COPE Polyclonal antibody
COPE Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11457-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, PC-3 cells, A375 cells, HeLa cells, A431 cells, MCF-7 cells |
Positive IHC detected in | human prostate cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
11457-1-AP targets COPE in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | COPE fusion protein Ag2024 |
Full Name | coatomer protein complex, subunit epsilon |
Calculated molecular weight | 34 kDa |
Observed molecular weight | 30-35 kDa |
GenBank accession number | BC017285 |
Gene symbol | COPE |
Gene ID (NCBI) | 11316 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for COPE antibody 11457-1-AP | Download protocol |
IHC protocol for COPE antibody 11457-1-AP | Download protocol |
IF protocol for COPE antibody 11457-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |