COPS4 Polyclonal antibody

COPS4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10464-1-AP

Synonyms

COPS4, CSN4, SGN4, Signalosome subunit 4

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Validation Data Gallery

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  WB analysis of mouse brain using 10464-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10464-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 10464-1-AP

  IP result of anti-COPS4 (IP:10464-1-AP, 4ug; Detection:10464-1-AP 1:300) with PC-3 cells lysate 1800ug.

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  IHC staining of human pancreas using 10464-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10464-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 10464-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10464-1-AP (COPS4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 10464-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10464-1-AP (COPS4 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10464-1-AP targets COPS4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COPS4 fusion protein Ag0735
• Full Name: COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis)
• Calculated Molecular Weight: 40; 46 kDa
• Observed Molecular Weight: 40 kDa, 46 kDa
• GenBank Accession Number: BC004302
• Gene Symbol: COPS4
• Gene ID (NCBI): 51138
• RRID: AB_2081630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an ess ential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. CSN4 gene encodes a component of CSN complex, COP9 signalosome complex subunit 4 (COPS4). The ubiquitin-proteasome system plays a major role in the rhythmic accumulation and turnover of molecular clock components. A recent study in Drosophila indicates that CSN lie in a common pathway leading to light-dependent degradation of clock protein Timeless (TIM).

Publications

Species	Application	Title
mouse	WB	Neuropsychiatr Dis Treat; COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein.; Authors - En Liang