Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 16628-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16628-1-AP
IP result of anti-COPS7A (IP:16628-1-AP, 4ug; Detection:16628-1-AP 1:5000) with HeLa cells lysate 1600 ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16628-1-AP (COPS7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 16628-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using COPS7A antibody (16628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using COPS7A antibody (16628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
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RAW 264.7 cells, mouse brain tissue, PC-12 cells, HeLa cells, rat brain tissue
Positive IP detected in
HeLa cells
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:5000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
16628-1-AP targets COPS7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
COPS7A is component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. COPS7A was consistently expressed in oligodendrocyte lineage cells, and was down-regulated during the peak period of myelination. COPS7A associated physically with Olig1, and it might thus serve as a novel negative regulator in oligodendrocytes differentiation.
Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 16628-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 16628-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16628-1-AP (COPS7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 16628-1-AP
IP result of anti-COPS7A (IP:16628-1-AP, 4ug; Detection:16628-1-AP 1:5000) with HeLa cells lysate 1600 ug.
IF/ICC Figures
IF Staining of MCF-7 using 16628-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using COPS7A antibody (16628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
COPS7A Polyclonal antibody
Catalog Number
16628-1-AP
Citations
-
Dilutions
WB : 1:1000-1:5000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
