COPS7A Polyclonal antibody
COPS7A Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16628-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | RAW 264.7 cells, PC-12 cells, HeLa cells |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
16628-1-AP targets COPS7A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | COPS7A fusion protein Ag9958 |
Full Name | COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis) |
Calculated molecular weight | 275 aa, 30 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC011789 |
Gene symbol | COPS7A |
Gene ID (NCBI) | 50813 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COPS7A is component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. COPS7A was consistently expressed in oligodendrocyte lineage cells, and was down-regulated during the peak period of myelination. COPS7A associated physically with Olig1, and it might thus serve as a novel negative regulator in oligodendrocytes differentiation.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for COPS7A antibody 16628-1-AP | Download protocol |
IHC protocol for COPS7A antibody 16628-1-AP | Download protocol |
IF protocol for COPS7A antibody 16628-1-AP | Download protocol |
IP protocol for COPS7A antibody 16628-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Mol Neurobiol Identification of the Interaction Between the Human Homologue of the Arabidopsis COP9 Signalosome Subunit 7a and Olig1. |