COPS7A Polyclonal antibody

COPS7A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16628-1-AP

Synonyms

COPS7A, CSN7A, DERP10, SGN7a, Signalosome subunit 7a



Tested Applications

Positive WB detected inRAW 264.7 cells, PC-12 cells, HeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16628-1-AP targets COPS7A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPS7A fusion protein Ag9958
Full Name COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis)
Calculated molecular weight 275 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC011789
Gene symbol COPS7A
Gene ID (NCBI) 50813
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPS7A is component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. COPS7A was consistently expressed in oligodendrocyte lineage cells, and was down-regulated during the peak period of myelination. COPS7A associated physically with Olig1, and it might thus serve as a novel negative regulator in oligodendrocytes differentiation.

Protocols

Product Specific Protocols
WB protocol for COPS7A antibody 16628-1-APDownload protocol
IHC protocol for COPS7A antibody 16628-1-APDownload protocol
IF protocol for COPS7A antibody 16628-1-APDownload protocol
IP protocol for COPS7A antibody 16628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Cell Mol Neurobiol

Identification of the Interaction Between the Human Homologue of the Arabidopsis COP9 Signalosome Subunit 7a and Olig1.

Authors - Jiazhen Zhou