COPS7A Polyclonal antibody

COPS7A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16628-1-AP

Synonyms

COPS7A, CSN7A, DERP10, SGN7a, Signalosome subunit 7a

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Validation Data Gallery

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  WB analysis using 16628-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16628-1-AP

  IP Result of anti-COPS7A (IP:16628-1-AP, 4ug; Detection:16628-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of human kidney using 16628-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16628-1-AP (COPS7A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16628-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16628-1-AP (COPS7A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 16628-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16628-1-AP (COPS7A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, PC-12 cells, HeLa cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

16628-1-AP targets COPS7A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COPS7A fusion protein Ag9958
• Full Name: COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis)
• Calculated molecular weight: 275 aa, 30 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC011789
• Gene symbol: COPS7A
• Gene ID (NCBI): 50813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPS7A is component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. COPS7A was consistently expressed in oligodendrocyte lineage cells, and was down-regulated during the peak period of myelination. COPS7A associated physically with Olig1, and it might thus serve as a novel negative regulator in oligodendrocytes differentiation.

Publications

Species	Application	Title
human	WB, IF	Cell Mol Neurobiol; Identification of the Interaction Between the Human Homologue of the Arabidopsis COP9 Signalosome Subunit 7a and Olig1.; Authors - Jiazhen Zhou