COPS8/COP9 Polyclonal antibody

COPS8/COP9 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10089-2-AP

Synonyms

COP9, COP9 homolog, COPS8, CSN8, hCOP9, SGN8, Signalosome subunit 8



Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

10089-2-AP targets COPS8/COP9 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPS8/COP9 fusion protein Ag0143
Full Name COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC003090
Gene symbol COPS8
Gene ID (NCBI) 10920
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPS8 is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases.

Protocols

Product Specific Protocols
WB protocol for COPS8/COP9 antibody 10089-2-APDownload protocol
IHC protocol for COPS8/COP9 antibody 10089-2-APDownload protocol
IP protocol for COPS8/COP9 antibody 10089-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Bioeng Biotechnol

Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.

Authors - Kaile Zhang
humanWB

Biochem Biophys Res Commun

Rig-G negatively regulates SCF-E3 ligase activities by disrupting the assembly of COP9 signalosome complex.

Authors - Xu Gui-Ping GP