|Positive WB detected in||mouse brain tissue, HepG2 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10089-2-AP targets COPS8/COP9 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COPS8/COP9 fusion protein Ag0143|
|Full Name||COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)|
|Calculated molecular weight||23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC003090|
|Gene ID (NCBI)||10920|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COPS8 is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases.
Front Bioeng Biotechnol
Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.
Biochem Biophys Res Commun
Rig-G negatively regulates SCF-E3 ligase activities by disrupting the assembly of COP9 signalosome complex.