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COPZ1 Polyclonal antibody

COPZ1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20440-1-AP

Synonyms

CGI 120, Coatomer subunit zeta 1, COPZ, COPZ1, HSPC181, Zeta 1 coat protein, Zeta 1 COP, zeta1 COP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPZ1 Polyclonal antibody (20440-1-AP)

    WB analysis using 20440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using COPZ1 Polyclonal antibody (20440-1-AP)

    WB analysis of Jurkat using 20440-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using COPZ1 Polyclonal antibody (20440-1-AP)

    WB analysis of K-562 using 20440-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COS-7 cells using COPZ1 Polyclonal antibody (20440-1-AP)

    WB analysis of COS-7 using 20440-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using COPZ1 Polyclonal antibody (20440-1-AP)

    IF Staining of HEK-293 using 20440-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 20440-1-AP (COPZ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOS-7 cells, K-562 cells, Jurkat cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20440-1-AP is COPZ1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPZ1 fusion protein Ag14234
Full Name coatomer protein complex, subunit zeta 1
Calculated molecular weight 177 aa, 20 kDa
Observed molecular weight 17-20 kDa
GenBank accession numberBC002849
Gene symbol COPZ1
Gene ID (NCBI) 22818
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COPZ1 antibody 20440-1-APDownload protocol
IF protocol for COPZ1 antibody 20440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genes (Basel)

A Comprehensive Pan-Cancer Analysis of the Regulation and Prognostic Effect of Coat Complex Subunit Zeta 1

Authors - Ye Hong
  • KD Validated
View Article