COPZ1 Polyclonal antibody

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  WB analysis using 20440-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20440-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 20440-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20440-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 20440-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 20440-1-AP (COPZ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

COPZ1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 20440-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC, ELISA

Synonyms

CGI 120, CGI-120, Coatomer subunit zeta 1, Coatomer subunit zeta-1, COPZ

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Tested Applications

• Positive WB detected in: COS-7 cells, K-562 cells, Jurkat cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

20440-1-AP targets COPZ1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COPZ1 fusion protein Ag14234
• Full Name: coatomer protein complex, subunit zeta 1
• Calculated Molecular Weight: 177 aa, 20 kDa
• Observed Molecular Weight: 17-20 kDa
• GenBank Accession Number: BC002849
• Gene Symbol: COPZ1
• Gene ID (NCBI): 22818
• RRID: AB_10733095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61923
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	MedComm (2020); Chemoproteomics enables identification of coatomer subunit zeta-1 targeted by a small molecule for enterovirus A71 inhibition; Authors - Xiaoyong Li
mouse	WB	Biochim Biophys Acta Gen Subj; COPZ1 regulates ferroptosis through NCOA4-mediated ferritinophagy in lung adenocarcinoma; Authors - Anbang Wu ; KD Validated
human	WB	Genes (Basel); A Comprehensive Pan-Cancer Analysis of the Regulation and Prognostic Effect of Coat Complex Subunit Zeta 1; Authors - Ye Hong ; KD Validated