COQ10A Polyclonal antibody

COQ10A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17812-1-AP

Synonyms

Coenzyme Q-binding protein COQ10 homolog A, mitochondrial

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Validation Data Gallery

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  WB analysis of human heart using 17812-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17812-1-AP (COQ10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17812-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17812-1-AP (COQ10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17812-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17812-1-AP (COQ10A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17812-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17812-1-AP (COQ10A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17812-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17812-1-AP (COQ10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

17812-1-AP targets COQ10A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COQ10A fusion protein Ag12126
• Full Name: coenzyme Q10 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 247 aa, 28 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC073923
• Gene Symbol: COQ10A
• Gene ID (NCBI): 93058
• RRID: AB_2081926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Front Pharmacol; Propofol Augments Paclitaxel-Induced Cervical Cancer Cell Ferroptosis In Vitro.; Authors - Meng-Yun Zhao
human	WB	Mol Med Rep; Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase.; Authors - Bingju Yan
rat	WB	Mol Med Rep; UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats.; Authors - Bingju Yan
human	WB	J Lipid Res; Human COQ10A and COQ10B are distinct lipid-binding START domain proteins required for coenzyme Q function.; Authors - Hui S Tsui