COQ6 Monoclonal antibody, PBS Only (Capture)

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  WB analysis of human heart using 67162-1-Ig (same clone as 67162-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67162-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 67162-1-Ig (same clone as 67162-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67162-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67162-1-Ig (same clone as 67162-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67162-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67162-1-Ig (same clone as 67162-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67162-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 67162-1-Ig (same clone as 67162-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 67162-1-Ig (same clone as 67162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 67162-1-Ig (same clone as 67162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67162-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67162-1-Ig (same clone as 67162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67162-1-Ig (same clone as 67162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67162-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50616-1

  Cytometric bead array standard curve of MP50616-1, COQ6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67162-1-PBS. Detection antibody: 67162-2-PBS. Standard:Ag28991. Range: 0.098-100 ng/mL.

COQ6 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 67162-1-PBS

Clone No.2F5B1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial, EC:1.14.13.-, Coenzyme Q10 monooxygenase 6, CGI-10, CGI 10

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Product Information

67162-1-PBS targets COQ6 as part of a matched antibody pair:

MP50616-1: 67162-1-PBS capture and 67162-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28991

  Product name: Recombinant human COQ6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 151-468 aa of BC014181

  Sequence: ALTKQLEAVSDRVTVLYRSKAIRYTWPCPFPMADSSPWVHITLGDGSTFQTKLLIGADGHNSGVRQAVGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEKFVDAVNSAFWSDADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGLGHAAEYVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHNTALLAATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK

• Full Name: coenzyme Q6 homolog, monooxygenase (S. cerevisiae)
• Calculated Molecular Weight: 468 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC014181
• Gene Symbol: COQ6
• Gene ID (NCBI): 51004
• RRID: AB_2882458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y2Z9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.