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  • KD/KO Validated

COQ7 Polyclonal antibody

COQ7 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15083-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

CAT5, CLK1, EC:1.14.99.60, Timing protein clk-1 homolog, Ubiquinone biosynthesis monooxygenase COQ7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse liver tissue, mouse heart tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15083-1-AP targets COQ7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COQ7 fusion protein Ag7161 Predict reactive species
Full Name coenzyme Q7 homolog, ubiquinone (yeast)
Calculated Molecular Weight 24 kDa
Observed Molecular Weight20~24 kDa
GenBank Accession NumberBC003185
Gene Symbol COQ7
Gene ID (NCBI) 10229
RRIDAB_2082207
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99807
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COQ7(Ubiquinone biosynthesis protein COQ7 homolog) catalyzes the production of coenzyme Q (CoQ) in mitochondria. The predicted protein contains 179 amino acids, is mostly helical, and contains an alpha-helical membrane insertion. It has a potential N-glycosylation site, a phosphorylation site for protein kinase C and another for casein kinase II, and 3 N-myristoylation sites. This protein has 2 isoforms produced by alternative splicing with MW 20-24 kDa.

Protocols

Product Specific Protocols
WB protocol for COQ7 antibody 15083-1-APDownload protocol
IHC protocol for COQ7 antibody 15083-1-APDownload protocol
IP protocol for COQ7 antibody 15083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Cell Biol

A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity.

Authors - Richard M Monaghan
humanWB

J Clin Invest

ADCK4 mutations promote steroid-resistant nephrotic syndrome through CoQ10 biosynthesis disruption.

Authors - Ashraf Shazia S
  • KD Validated
mouseWB

Nat Commun

Hepatic HuR modulates lipid homeostasis in response to high-fat diet.

Authors - Zhuojun Zhang
mouseWB

EMBO Mol Med

β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.

Authors - Agustín Hidalgo-Gutiérrez
mouseWB

Redox Biol

The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.

Authors - Pilar González-García
mouseWB

Acta Pharmacol Sin

Dysregulation of iron homeostasis and methamphetamine reward behaviors in Clk1-deficient mice.

Authors - Peng-Ju Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Ying (Verified Customer) (04-26-2021)

It's super clean and works great.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse and human fibroblasts, mouse tissues and human muscle biopsy samples
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