CORO2A Polyclonal antibody

CORO2A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10119-1-AP

Synonyms

CLIPINB, CORO2A, Coronin 2A, CRN5, IR10, WD repeat containing protein 2, WDR2



Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman skin cancer tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10119-1-AP targets CORO2A in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CORO2A fusion protein Ag0164
Full Name coronin, actin binding protein, 2A
Calculated molecular weight 60 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000010
Gene symbol CORO2A
Gene ID (NCBI) 7464
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CORO2A (Coronin 2A), also named as CLIPINB, is a member of the 'short' coronin subfamily containing a single WD40-repeat domain. It has been shown that the expression of CORO2A is up-regulated in human colon cancer. CORO2A can interact with MAPK14 and PRMT5. The calculated MW of CORO2A is 60 kDa, while 10119-1-AP antibody detects 48 kDa protein which is similar to the paper published. (PMID: 26373535)

Protocols

Product Specific Protocols
WB protocol for CORO2A antibody 10119-1-APDownload protocol
IHC protocol for CORO2A antibody 10119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols