CORO2B Polyclonal antibody

CORO2B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13802-1-AP

Synonyms

Protein FC96, KIAA0925, Coronin-like protein C, Coronin-2B, Coronin 2B

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Validation Data Gallery

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  WB analysis of Jurkat using 13802-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13802-1-AP (CORO2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13802-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13802-1-AP (CORO2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 13802-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13802-1-AP (CORO2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 13802-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13802-1-AP (CORO2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13802-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13802-1-AP (CORO2B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13802-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13802-1-AP (CORO2B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue
• Positive IHC detected in: human ovary cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13802-1-AP targets CORO2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CORO2B fusion protein Ag4793
• Full Name: coronin, actin binding protein, 2B
• Calculated Molecular Weight: 475 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC026335
• Gene Symbol: CORO2B
• Gene ID (NCBI): 10391
• RRID: AB_2087042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.