Tested Applications
| Positive WB detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
Product Information
10611-2-AP targets COX10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0930 Product name: Recombinant human COX10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC006394 Sequence: MAASPHTLSSRLLTGCVGGSVWYLERRTIQDSPHKFLHLLRNVNKQWITFQHFSFLKRMYVTQLNRSHNQQVRPKPEPVASPFLEKTSSGQAKAEIYEMRPLSPPSLSLSRKPNEKELIELEPDSVIEDSIDVGKETKEEKRWKEMKLQVYDLPGILAQLSKIKLTALVVSTTAAGFALAPGPFDWPCFLLTSVGTGLASCAANSINQFFEVPFDSNMNRTKNRPLVRGQISPLLAVSFATCCAVPGVAILTLGVNPLTGALGLFNIFLYTCCYTPLKRISIANTWVGAVVGAIPPVMGW Predict reactive species |
| Full Name | COX10 homolog, cytochrome c oxidase assembly protein, heme A: farnesyltransferase (yeast) |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC006394 |
| Gene Symbol | COX10 |
| Gene ID (NCBI) | 1352 |
| RRID | AB_2084833 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12887 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytochrome c oxidase (COX) is the terminal enzyme in the electron transfer chain located in the mitochondrial inner membrane. It catalyzes the electron transfer from reduced cytochrome c to oxygen. COX10 is predicted to contain 7-9 transmembrane domains, and it is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes heme A:farnesyltransferase, which is required for the expression of functional COX and functions in the maturation of the heme A prosthetic group of COX. In addition, COX10 may play a certain role in the development and progression of azoospermia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COX10 antibody 10611-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation. | ||
Transl Res Skeletal Muscle MiR-210 Expression is Associated with Mitochondrial Function in Peripheral Artery Disease Patients. | ||
EMBO Mol Med Expression of the alternative oxidase complements cytochrome c oxidase deficiency in human cells.
| ||
Free Radic Biol Med Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3. | ||
Glia Deficient mitochondrial respiration in astrocytes impairs trace fear conditioning and increases naloxone-precipitated aversion in morphine-dependent mice.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kateryna (Verified Customer) (01-16-2025) | The antibody worked very well in Western blot (SDS-PAGE, nitrocellulose membrane, 5% non-fat dry milk blocking). The antibody was validated on knockout mice lacking COX10 in brain astrocytes (Aldh1l1-Cre/ERT2::Cox10fl/+).
 |
