Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11498-1-AP targets COX11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2033 Product name: Recombinant human COX11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-276 aa of BC005895 Sequence: MGGLWRPGWRCVPFCGWRWIHPGSPTRAAERVEPFLRPEWSGTGGAERGLRWLGTWKRCSLRARHPALQPPRRPKSSNPFTRAQEEERRRQNKTTLTYVAAVAVGMLGASYAAVPLYRLYCQTTGLGGSAVAGHASDKIENMVPVKDRIIKISFNADVHASLQWNFRPQQTEIYVVPGETALAFYRVKNPTDKPVIGISTYNIVPFEAGQYFNKIQCFCFEEQRLNPQEEVDMPVFFYIDPEFAEDPRMIKVDLITLSYTFFEAKEGHKLPVPGYN Predict reactive species |
| Full Name | COX11 homolog, cytochrome c oxidase assembly protein (yeast) |
| Calculated Molecular Weight | 276 aa, 31 kDa |
| Observed Molecular Weight | 25-31 kDa |
| GenBank Accession Number | BC005895 |
| Gene Symbol | COX11 |
| Gene ID (NCBI) | 1353 |
| RRID | AB_10598173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6N1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for COX11 antibody 11498-1-AP | Download protocol |
| IP protocol for COX11 antibody 11498-1-AP | Download protocol |
| WB protocol for COX11 antibody 11498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The COX11 Polyclonal antibody (Cat# 11498-1-AP) has 6 citations published in Life Sciences, Scientific Reports, Journal of Molecular Biology, Journal of Neuroscience, Neuroscience Letters, and Nature Communications. Research fields span neuroinflammation and memory deficits, copper metabolism in osteosarcoma, mitochondrial cytochrome c oxidase biogenesis, Golgi-dependent copper homeostasis in synaptic development, RANBP2-related encephalopathy, and the human mitochondrial proteome interactome.
| Species | Application | Title |
|---|---|---|
Life Sci Steroidal alkaloid H89712 ameliorates neuroinflammation and memory deficits: Enhancing cerebral oxidative phosphorylation in APP/PS1 mice. | ||
Sci Rep Comprehensive analysis of copper-metabolism-related genes about prognosis and immune microenvironment in osteosarcoma | ||
J Mol Biol COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria. | ||
J Neurosci Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content. | ||
Neurosci Lett RANBP2 mutation causing autosomal dominant acute necrotizing encephalopathy attenuates its interaction with COX11. | ||
