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Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27712-1-AP targets COX14 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24036 Product name: Recombinant human C12orf62 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-57 aa of BC007849 Sequence: MPTGKQLADIGYKTFSTSMMLLTVYGGYLCSVRVYHYFQWRRAQRQAAEEQKTSGIM Predict reactive species |
| Full Name | Cytochrome c oxidase assembly protein COX14 |
| Observed Molecular Weight | 7-12 kDa |
| GenBank Accession Number | BC007849 |
| Gene Symbol | C12orf62 |
| Gene ID (NCBI) | 84987 |
| RRID | AB_3742250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96I36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COX14 (Cytochrome c Oxidase Assembly Factor 14) is a protein that functions as a crucial assembly factor for mitochondrial complex IV (cytochrome c oxidase, COX), the terminal enzyme of the electron transport chain .
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COX14 antibody 27712-1-AP | Download protocol |
| WB protocol for COX14 antibody 27712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
