COX15 Polyclonal antibody

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  WB analysis using 11441-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11441-1-AP (COX15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 11441-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11441-1-AP (COX15 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 11441-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11441-1-AP (COX15 antibody) at dilution of 1:100 (under 40x lens).

COX15 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11441-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

COX15

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

11441-1-AP targets COX15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2004

  Product name: Recombinant human COX15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-410 aa of BC002382

  Sequence: MQRLLFPPLRALKGRQYLPLLAPRAAPRAQCDCIRRPLRPGQYSTISEVALQSGRGTVSLPSKAAERVVGRWLLVCSGTVAGAVILGGVTRLTESGLSMVDWHLIKEMKPPTSQEEWEAEFQRYQQFPEFKILNHDMTLTEFKFIWYMEYSHRMWGRLVGLVYILPAAYFWRKGWLSRGMKGRVLALCGLVCFQGLLGWYMVKSGLEEKSDSHDIPRVSQYRLAAHLGSALVLYCASLWTSLSLLLPPHKLPETHQLLQLRRFAHGTAGLVFLTALSGAFVAGLDAGLVYNSFPKMGESWIPEDLFTFSPILRNVFENPTMVQFDHRILGITSVTAITVLYFLSRRIPLPRRTKMAAVTLLALAYTQVGLGISTLLMYVPTPLAATHQSGSLALLTGALWLMNELRRVPK

• Full Name: COX15 homolog, cytochrome c oxidase assembly protein (yeast)
• Calculated Molecular Weight: 410 aa, 46 kDa
• Observed Molecular Weight: 44-55 kDa
• GenBank Accession Number: BC002382
• Gene Symbol: COX15
• Gene ID (NCBI): 1355
• RRID: AB_10792251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7KZN9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX15 belongs to the COX15/CtaA family. It may be involved in the biosynthesis of heme A. COX15 has 2 isoforms COX15.1 and COX15.2. The MW in SDS-PAGE is between 44kd and 55kd.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Theranostics; AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.; Authors - Liangjun Xia
mouse	WB	Int J Mol Sci; Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling; Authors - Jana Key
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure.; Authors - Qianqian Guo
mouse	WB	J Nanobiotechnology; Modulation of alveolar macrophage and mitochondrial fitness by medicinal plant-derived nanovesicles to mitigate acute lung injury and viral pneumonia; Authors - Lusha Ye