COX5A Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86938-1-RR (same clone as 86938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86938-1-RR (COX5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86938-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86938-1-RR (same clone as 86938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86938-1-RR (COX5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86938-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 86938-1-RR (same clone as 86938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86938-1-RR (COX5A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86938-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 86938-1-RR (same clone as 86938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86938-1-RR (COX5A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86938-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 86938-1-RR (same clone as 86938-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COX5A antibody (86938-1-RR, Clone: 251697F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86938-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86938-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86938-1-RR against Human COX5A were performed. The affinity constant is 0.804 nM.

COX5A Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86938-1-PBS

Clone No.251697F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

COX VA, COX5, COXVA

---

Product Information

86938-1-PBS targets COX5A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2005

  Product name: Recombinant human COX5A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-150 aa of BC024240

  Sequence: MLGAALRRCAVAATTRADPRGLLHSARTPGPAVAIQSVRCYSHGSQETDEEFDARWVTYFNKPDIDAWELRKGINTLVTYDMVPEPKIIDAALRACRRLNDFASTVRILEVVKDKAGPHKEIYPYVIQELRPTLNELGISTPEELGLDKV

• Full Name: cytochrome c oxidase subunit Va
• Calculated Molecular Weight: 150 aa, 17 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC024240
• Gene Symbol: COX5A
• Gene ID (NCBI): 9377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20674
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

COX5A(Cytochrome c oxidase subunit 5A, mitochondrial)is also named COXVA and belongs to the cytochrome c oxidase subunit 5A family. This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).