|Positive WB detected in||HeLa cells, human heart tissue, human liver tissue, L02 cells, mouse liver tissue, rat liver tissue|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11418-2-AP targets COX5B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COX5B fusion protein Ag1986|
|Full Name||cytochrome c oxidase subunit Vb|
|Calculated molecular weight||129aa,14 kDa|
|Observed molecular weight||14 kDa|
|GenBank accession number||BC006229|
|Gene ID (NCBI)||1329|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)
Biochim Biophys Acta
Analysis of the mitochondrial proteome in multiple sclerosis cortex.
Biochim Biophys Acta
The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis.
J Biol Chem
Acquisition of temozolomide chemoresistance in gliomas leads to remodeling of mitochondrial electron transport chain.
Cell Death Dis
O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.
Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle.
Mitochondrial respiratory-chain adaptations in macrophages contribute to antibacterial host defense.