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  • KD/KO Validated

COX5B Polyclonal antibody

COX5B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11418-2-AP

Synonyms

COX5B, COXVB



Tested Applications

Positive WB detected inHeLa cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11418-2-AP targets COX5B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX5B fusion protein Ag1986
Full Name cytochrome c oxidase subunit Vb
Calculated molecular weight 129 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC006229
Gene symbol COX5B
Gene ID (NCBI) 1329
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)

Protocols

Product Specific Protocols
WB protocol for COX5B antibody 11418-2-APDownload protocol
IHC protocol for COX5B antibody 11418-2-APDownload protocol
IF protocol for COX5B antibody 11418-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Mechanism of super-assembly of respiratory complexes III and IV.

Authors - Sara Cogliati
humanWB

Cell

Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.

Authors - Ricarda Richter-Dennerlein
  • KD Validated
mouseWB

Nat Immunol

Mitochondrial respiratory-chain adaptations in macrophages contribute to antibacterial host defense.

Authors - Johan Garaude
humanWB

Cell Metab

Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle.

Authors - Chiara Greggio
humanWB

Mol Cell

Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery

Authors - Jasmin Adriana Schafer
humanWB

Cell Death Dis

O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.

Authors - Weicheng Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Süleyman (Verified Customer) (02-07-2022)

HeLa cell line is used and even though it has some unspecific bands but it is very specific and the unspecific bands are very distant. Recommended!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa
COX5B Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:11418-2-AP)