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COX5B Antibody

COX5B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11418-2-AP

Synonyms

COX5B, COXVB



Tested Applications

Positive WB detected inHeLa cells, human heart tissue, human liver tissue, L02 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11418-2-AP targets COX5B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX5B fusion protein Ag1986
Full Name cytochrome c oxidase subunit Vb
Calculated molecular weight 129aa,14 kDa
Observed molecular weight 14 kDa
GenBank accession number BC006229
Gene symbol COX5B
Gene ID (NCBI) 1329
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)

Protocols

Product Specific Protocols
WB protocol for COX5B antibody 11418-2-APDownload protocol
IHC protocol for COX5B antibody 11418-2-APDownload protocol
IF protocol for COX5B antibody 11418-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Biochim Biophys Acta

Analysis of the mitochondrial proteome in multiple sclerosis cortex.

Authors - Broadwater Laurie L
humanWB

Biochim Biophys Acta

The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis.

Authors - Isotta Lorenzi
humanWB

J Biol Chem

Acquisition of temozolomide chemoresistance in gliomas leads to remodeling of mitochondrial electron transport chain.

Authors - Oliva Claudia R CR
humanWB

Cell Death Dis

O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.

Authors - Weicheng Wu
humanWB

Cell Metab

Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle.

Authors - Chiara Greggio
mouseWB

Nat Immunol

Mitochondrial respiratory-chain adaptations in macrophages contribute to antibacterial host defense.

Authors - Johan Garaude