COX7C Polyclonal antibody

• Featured Product
• KD/KO Validated

COX7C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11411-2-AP

Synonyms

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Validation Data Gallery

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  WB analysis of mouse ovary using 11411-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11411-2-AP (COX7C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11411-2-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11411-2-AP (COX7C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11411-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11411-2-AP (COX7C antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 11411-2-AP

  Immunofluorescent analysis of MCF-7 cells, using COX7C antibody 11411-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse ovary tissue, human skeletal muscle tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Product Information

11411-2-AP targets COX7C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX7C fusion protein Ag1989
• Full Name: cytochrome c oxidase subunit VIIc
• Calculated Molecular Weight: 63 aa, 7 kDa
• Observed Molecular Weight: 15~28 kDa
• GenBank Accession Number: BC007498
• Gene Symbol: COX7C
• Gene ID (NCBI): 1350
• RRID: AB_2085713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. COX7C (Cytochrome c oxidase subunit 7C, mitochondrial) is one of the subunits, catalyzing the elctron transfer from reduced cytochrome c to molecule oxygen. This protein belongs to the cytochrome c oxidase VIIc family. The calculated molecular weight of COX7C is 7 kDa, but due to the presence of the complex, a complex form of 28 kDa may also be observed.

Publications

Species	Application	Title
human	WB	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu
human	WB	J Biol Chem; PMID: 20870728; Authors - Oliva Claudia R CR
human	WB	Cancer Sci; Urea transport B gene induces melanoma B16 cell death via activation of p53 and mitochondrial apoptosis.; Authors - Lianqin Liu
human	IF	J Neuropathol Exp Neurol; Altered Mitochondria, Protein Synthesis Machinery, and Purine Metabolism Are Molecular Contributors to the Pathogenesis of Creutzfeldt-Jakob Disease.; Authors - Belén Ansoleaga
mouse	WB,IF	Front Pharmacol; Effect of Dl-3-n-butylphthalide on mitochondrial Cox7c in models of cerebral ischemia/reperfusion injury; Authors - Jingjing Jia ; KD Validated
mouse	WB	Nat Commun; ZHX2 emerges as a negative regulator of mitochondrial oxidative phosphorylation during acute liver injury; Authors - Yankun Zhang