Carboxypeptidase A5 Polyclonal antibody

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  WB analysis of PC-3 using 13731-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13731-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 13731-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 13731-1-AP

  Immunofluorescent analysis of PC-3 cells using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Carboxypeptidase A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13731-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CPA5, EC:3.4.17.-

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human testis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13731-1-AP targets Carboxypeptidase A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4755

  Product name: Recombinant human CPA5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 126-436 aa of BC039362

  Sequence: RLERSTNSFSYSSYHTLEEIYSWIDNFVMEHSDIVSKIQIGNSFENQSILVLKFSTGGSRHPAIWIDTGIHSREWITHATGIWTANKIVSDYGKDRVLTDILNAMDIFIELVTNPDGFAFTHSMNRLWRKNKSIRPGIFCIGVDLNRNWKSGFGGNGSNSNPCSETYHGPSPQSEPEVAAIVNFITAHGNFKALISIHSYSQMLMYPYGRLLEPVSNQRELYDLAKDAVEALYKVHGIEYIFGSISTTLYVASGITVDWAYDSGIKYAFSFELRDTGQYGFLLPATQIIPTAQETWMALRTIMEHTLNHPY

• Full Name: carboxypeptidase A5
• Calculated Molecular Weight: 436 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC039362
• Gene Symbol: CPA5
• Gene ID (NCBI): 93979
• RRID: AB_2261098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WXQ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carboxypeptidase A5 (CPA5) is a member of the carboxypeptidase family. CPA5 was identified during a genome-wide search for human metallocarboxypeptidase-like genes with sequence similarity to other carboxypeptidases.