CPOX Polyclonal antibody

• Featured Product
• KD/KO Validated

CPOX Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12211-1-AP

Synonyms

Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP

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Validation Data Gallery

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  WB analysis using 12211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12211-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12211-1-AP

  IP result of anti-CPOX (IP:12211-1-AP, 4ug; Detection:12211-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12211-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12211-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, Jurkat cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

12211-1-AP targets CPOX in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPOX fusion protein Ag2858
• Full Name: coproporphyrinogen oxidase
• Calculated Molecular Weight: 454 aa, 50 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC023551
• Gene Symbol: CPOX
• Gene ID (NCBI): 1371
• RRID: AB_2084233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.

Publications

Species	Application	Title
human	WB, IF	J Cell Biol; Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.; Authors - Yumiko Oshima
human	IHC	Neuro Oncol; Enhanced expression of coproporphyrinogen oxidase in malignant brain tumors: CPOX expression and 5-ALA-induced fluorescence.; Authors - Takahashi Kenkichi K
human,mouse	WB	Biochem Pharmacol; Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis.; Authors - Katalin Módis ; KD Validated
human	IHC	Front Med (Lausanne); Heme Biosynthesis Factors and 5-ALA Induced Fluorescence: Analysis of mRNA and Protein Expression in Fluorescing and Non-fluorescing Gliomas.; Authors - Mario Mischkulnig
human	IHC	Photodiagnosis Photodyn Ther; Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis.; Authors - Kumi Ogihara
human	IHC	Photodiagnosis Photodyn Ther; Protoporphyrinogen oxidase is involved in the fluorescence intensity of 5-aminolevulinic acid-mediated laser-based photodynamic endoscopic diagnosis for early gastric cancer.; Authors - Hiroki Kurumi