Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells, mouse liver tissue, Jurkat cells, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12211-1-AP targets CPOX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2858 Product name: Recombinant human CPOX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC023551 Sequence: MALQLGRLSSGPCWLVARGGCGGPRAWSQCGGGGLRAWSQRSAAGRVCRPPGPAGTEQSRGLGHGSTSRGGPWVGTGLAAALAGLVGLATAAFGHVQRAEMLPKTSGTRATSLGRPEEEEDELAHRCSSFMAPPVTDLGELRRRPGDMKTKMELLILETQAQVCQALAQVDGGANFSVDRWERKEGGGGISCVLQDGCVFEKAGVSISVVHGNLSEEAAKQMRSRGKVLKTKDGKLPFCAMGVSSVIHPKNPHAPTIHFNYRYFEVEEADGNKQWWFGGGCDLTPTYLNQEDAVHFHRTLKEA Predict reactive species |
| Full Name | coproporphyrinogen oxidase |
| Calculated Molecular Weight | 454 aa, 50 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC023551 |
| Gene Symbol | CPOX |
| Gene ID (NCBI) | 1371 |
| RRID | AB_2084233 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36551 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CPOX antibody 12211-1-AP | Download protocol |
| IP protocol for CPOX antibody 12211-1-AP | Download protocol |
| WB protocol for CPOX antibody 12211-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CPOX Polyclonal antibody (Cat# 12211-1-AP) has 11 citations. These appear in journals including Nature, Kidney International, Nature Communications, Neuro-Oncology, Cell Reports, Biochemical Pharmacology, Journal of Cell Biology, Frontiers in Medicine, Photodiagnosis and Photodynamic Therapy, and Bioactive Materials. Research fields span heme biosynthesis, mitochondrial biology and metabolism, photodynamic diagnosis/therapy, and cancer (glioma, lung, gastric, and brain tumors). Applications cited include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Kidney Int Non-invasive quantitative assessment of kidney injury using near-infrared autofluorescence imaging. | ||
Nat Commun YBX1 Confers immunosuppressive bone metastatic traits in non-small cell lung cancer. | ||
Neuro Oncol Enhanced expression of coproporphyrinogen oxidase in malignant brain tumors: CPOX expression and 5-ALA-induced fluorescence. | ||
Cell Rep The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism. | ||
Biochem Pharmacol Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis.
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