CPS1 Recombinant monoclonal antibody, PBS Only

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  WB analysis of HeLa using 84277-1-RR (same clone as 84277-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 84277-1-RR (same clone as 84277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84277-1-RR (same clone as 84277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84277-1-RR (CPS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84277-1-RR (same clone as 84277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84277-1-RR (CPS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84277-1-RR (same clone as 84277-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (84277-1-RR, Clone: 241198F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

CPS1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84277-1-PBS

Clone No.241198F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:6.3.4.16, CPSase I, Carbamoyl-phosphate synthetase I, Carbamoyl-phosphate synthase [ammonia], mitochondrial, 241198F1

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Product Information

84277-1-PBS targets CPS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: carbamoyl-phosphate synthetase 1, mitochondrial
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 140-165 kDa
• GenBank Accession Number: BC140943
• Gene Symbol: CPS1
• Gene ID (NCBI): 1373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P31327
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.