CPSF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CPSF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11031-1-AP

Synonyms

CPSF 160 kDa subunit, CPSF1, CPSF160, HSU37012, P/cl.18

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 11031-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11031-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11031-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11031-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells, human brain tissue, HeLa cells
• Positive IHC detected in: mouse brain tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

11031-1-AP targets CPSF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPSF1 fusion protein Ag1489
• Full Name: cleavage and polyadenylation specific factor 1, 160kDa
• Calculated Molecular Weight: 160 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC017232
• Gene Symbol: CPSF1
• Gene ID (NCBI): 29894
• RRID: AB_2245276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis.

Publications

Species	Application	Title
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB	J Biosci; Targeting cleavage and polyadenylation specific factor 1 via shRNA inhibits cell proliferation in human ovarian cancer.; Authors - Beiguang Zhang ; KD Validated