CPSF2 Polyclonal antibody

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• CPSF2 Polyclonal antibody (17739-1-AP)

CPSF2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17739-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IP, ELISA

Synonyms

CPSF 100 kDa subunit, CPSF100, KIAA1367

• 

  WB analysis using 17739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 17739-1-AP

  IP result of anti-CPSF2 (IP:17739-1-AP, 4ug; Detection:17739-1-AP 1:3000) with HeLa cells lysate 1480 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17739-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17739-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C2C12 cells, COLO 205 cells, COS-7 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17739-1-AP targets CPSF2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11950

  Product name: Recombinant human CPSF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 433-782 aa of BC070095

  Sequence: SAHKTKHDLMMKGEGSRKGSFFKQAKKSYPMFPAPEERIKWDEYGEIIKPEDFLVPELQATEEEKSKLESGLTNGDEPMDQDLSDVPTKCISTTESIEIKARVTYIDYEGRSDGDSIKKIINQMKPRQLIIVHGPPEASQDLAECCRAFGGKDIKVYMPKLHETVDATSETHIYQVRLKDSLVSSLQFCKAKDAELAWIDGVLDMRVSKVDTGVILEEGELRDDGEDSEMQVEAPSDSSVIAQQKAMKSLFGDDEKETGEESEIIPTLEPLPPHEVPGHQSVFMNEPRLSDFKQVLLREGIQAEFVGGVLVCNNQVAVRRTETGRIGLEGCLCQDFYRIRDLLYEQYAIV

• Full Name: cleavage and polyadenylation specific factor 2, 100kDa
• Calculated Molecular Weight: 782 aa, 88 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC070095
• Gene Symbol: CPSF2
• Gene ID (NCBI): 53981
• RRID: AB_2084368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2I0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CPSF2 polyclonal antibody (Cat# 17739-1-AP) has 2 citations. These appear in Cell Discovery (2024) and Neuron (2022), both with an impact factor of 16.9. The cited research spans glioblastoma stem cell biology involving alternative polyadenylation and neurodegeneration related to premature transcription termination, placing the antibody's use in oncology and neuroscience fields.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Discov; RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation; Authors - Peng Lin
		Neuron; Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.; Authors - Geneva R LaForce