CPSF2 Polyclonal antibody

CPSF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17739-1-AP

Synonyms

KIAA1367, CPSF100, CPSF 100 kDa subunit

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Validation Data Gallery

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  WB analysis of HeLa using 17739-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17739-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17739-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17739-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17739-1-AP

  IP result of anti-CPSF2 (IP:17739-1-AP, 4ug; Detection:17739-1-AP 1:1000) with HeLa cells lysate 4000ug.

• 

  IHC staining of human brain using 17739-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17739-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17739-1-AP

  Immunofluorescent analysis of HepG2 cells using 17739-1-AP (CPSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

17739-1-AP targets CPSF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPSF2 fusion protein Ag11950
• Full Name: cleavage and polyadenylation specific factor 2, 100kDa
• Calculated Molecular Weight: 782 aa, 88 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC070095
• Gene Symbol: CPSF2
• Gene ID (NCBI): 53981
• RRID: AB_2084368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	CoIP	Neuron; Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.; Authors - Geneva R LaForce
human	WB	Cell Discov; RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation; Authors - Peng Lin