CPSF3 Polyclonal antibody

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  WB analysis using 11609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11609-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11609-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:700.

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  IHC staining of mouse kidney using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CPSF3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11609-1-AP

Publications(6)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

mRNA 3'-end-processing endonuclease CPSF-73, EC:3.1.27.-, CPSF73, CPSF 73 kDa subunit, CPSF 73

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Tested Applications

• Positive WB detected in: C2C12 cells, HEK-293 cells, HepG2 cells, human brain tissue, HeLa cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse kidney tissue, human placenta tissue, human thyroid cancer tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below

Product Information

11609-1-AP targets CPSF3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPSF3 fusion protein Ag2173
• Full Name: cleavage and polyadenylation specific factor 3, 73kDa
• Calculated Molecular Weight: 684 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC020211
• Gene Symbol: CPSF3
• Gene ID (NCBI): 51692
• RRID: AB_2292188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKF6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPSF3 (cleavage and polyadenylation specificity factor 3) is involved in human disease and is the putative target of benzoxaboroles in trypanosomatid parasites and Cryptosporidium infection. CPSF3 has also been associated with cancer malignancy in that CPSF3 induces cell cycle arrest in hepatocellular carcinoma and promotes lung adenocarcinoma cell proliferation and metastasis (PMID: 38718887). CPSF3 has been implicated in various cancers, including lung cancer, colorectal, prostate, and triple-negative breast cancers (PMID: 37627085).

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.; Authors - Kaili Luo ; KD Validated
human	WB	Cancers (Basel); CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma; Authors - Ying Huang
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
pig	WB	Cell Mol Life Sci; Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs; Authors - Xiaolong Yuan
human	WB	Cell Chem Biol; Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3; Authors - Ye Tao
human	WB	Cell Discov; RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation; Authors - Peng Lin ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (06-12-2024)

Works perfectly

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cell line

CPSF3 Antibody Western Blot validation (1:1000 dilution) in HeLa cell line (Cat no:11609-1-AP)