CPSF7 Polyclonal antibody
CPSF7 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 55195-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, mouse brain tissue |
Positive IHC detected in | human gliomas tissue, human skin tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
CoIP | See 1 publications below |
Product Information
55195-1-AP targets CPSF7 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | pre-mRNA cleavage factor I, 59 kDa subunit |
Calculated molecular weight | 56 kDa |
Observed molecular weight | 52 kDa, 56 kDa |
GenBank accession number | NM_001136040 |
Gene symbol | FLJ12529 |
Gene ID (NCBI) | 79869 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.
Protocols
Product Specific Protocols | |
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WB protocol for CPSF7 antibody 55195-1-AP | Download protocol |
IHC protocol for CPSF7 antibody 55195-1-AP | Download protocol |
IF protocol for CPSF7 antibody 55195-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Mol Cell Biol U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors |