CPSF7 Polyclonal antibody

CPSF7 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55195-1-AP

Synonyms

CPSF 59 kDa subunit, CPSF7, FLJ12529



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, human skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55195-1-AP targets CPSF7 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name pre-mRNA cleavage factor I, 59 kDa subunit
Calculated molecular weight 56 kDa
Observed molecular weight 52 kDa, 56 kDa
GenBank accession numberNM_001136040
Gene symbol FLJ12529
Gene ID (NCBI) 79869
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.

Protocols

Product Specific Protocols
WB protocol for CPSF7 antibody 55195-1-APDownload protocol
IHC protocol for CPSF7 antibody 55195-1-APDownload protocol
IF protocol for CPSF7 antibody 55195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols