CPSF7 Polyclonal antibody

CPSF7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55195-1-AP

Synonyms

CPSF 59 kDa subunit, CPSF7, FLJ12529

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Validation Data Gallery

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  WB analysis using 55195-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55195-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55195-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55195-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55195-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human skin tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• CoIP: See 1 publications below

Product Information

55195-1-AP targets CPSF7 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: pre-mRNA cleavage factor I, 59 kDa subunit
• Calculated molecular weight: 56 kDa
• Observed molecular weight: 52 kDa, 56 kDa
• GenBank accession number: NM_001136040
• Gene symbol: FLJ12529
• Gene ID (NCBI): 79869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.

Publications

Species	Application	Title
human	CoIP	J Mol Cell Biol; U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors; Authors - Zhijie Hu