Validation Data Gallery
|Positive WB detected in||A549 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66039-1-Ig is CPT1A Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CPT1A fusion protein Ag7745|
|Full Name||carnitine palmitoyltransferase 1A (liver)|
|Calculated molecular weight||88 kDa|
|Observed molecular weight||86 kDa|
|GenBank accession number||BC000185|
|Gene ID (NCBI)||1374|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.
|Product Specific Protocols|
|WB protocol for CPT1A antibody 66039-1-Ig||Download protocol|
|IHC protocol for CPT1A antibody 66039-1-Ig||Download protocol|
|IP protocol for CPT1A antibody 66039-1-Ig||Download protocol|
|FC protocol for CPT1A antibody 66039-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mohammad (Verified Customer) (04-29-2019)
working well, Without any non-specific band