CPT1A Monoclonal antibody

• Featured Product
• KD/KO Validated

CPT1A Monoclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

5D8G9

Reviews (1)

Cat no : 66039-1-Ig

Synonyms

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1

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Validation Data Gallery

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  WB analysis of A549 using 66039-1-Ig

  WB result of CPT1A antibody (66039-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPT1A transfected A549 cells.

• 

  IP experiment of MCF-7 using 66039-1-Ig

  IP Result of anti-CPT1A (IP:66039-1-Ig, 4ug; Detection:66039-1-Ig 1:500) with MCF-7 cells lysate 1200ug.

• 

  IHC staining of human breast cancer using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 66039-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug CPT1A antibody (66039-1-Ig, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

The immunogen of 66039-1-Ig is CPT1A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CPT1A fusion protein Ag7745
• Full Name: carnitine palmitoyltransferase 1A (liver)
• Calculated molecular weight: 88 kDa
• Observed molecular weight: 86 kDa
• GenBank accession number: BC000185
• Gene symbol: CPT1A
• Gene ID (NCBI): 1374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Publications

Species	Application	Title
human	IHC	Mol Biosyst; Metabolomics reveal mitochondrial and fatty acid metabolism disorders that contribute to the development of DKD in T2DM patients.; Authors - Ling Li
rat	WB,IF	Cell Death Discov; The lncRNA MIAT regulates CPT-1a mediated cardiac hypertrophy through m6A RNA methylation reading protein Ythdf2.; Authors - Yiqing Yang
mouse	WB	BMC Dev Biol; Etomoxir regulates the differentiation of male germ cells by specifically reducing H3K27ac level.; Authors - Yushan Xu
mouse	WB,IHC	PLoS One; Circadian and Dopaminergic Regulation of Fatty Acid Oxidation Pathway Genes in Retina and Photoreceptor Cells.; Authors - Patrick Vancura
mouse	WB,IHC	Food Funct; Hawk tea prevents high-fat diet-induced obesity in mice by activating the AMPK/ACC/SREBP1c signaling pathways and regulating the gut microbiota.; Authors - Wei Tao
mouse	WB	Food Funct; PMID: 32613952; Authors - Shuli Man

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mohammad (Verified Customer) (04-29-2019)

working well, Without any non-specific band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse hippocampus