CPT1A Monoclonal antibody

• Featured Product
• KD/KO Validated

CPT1A Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

25

CloneNo.

5D8G9

Reviews (1)

Cat no : 66039-1-Ig

Synonyms

CPT-1A, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform, Carnitine O-palmitoyltransferase 1, liver isoform

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Validation Data Gallery

• 

  WB analysis of A549 using 66039-1-Ig

  WB result of CPT1A antibody (66039-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPT1A transfected A549 cells.

• 

  IP experiment of MCF-7 using 66039-1-Ig

  IP result of anti-CPT1A (IP:66039-1-Ig, 4ug; Detection:66039-1-Ig 1:500) with MCF-7 cells lysate 1200ug.

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  IHC staining of human breast cancer using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 66039-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CPT1A antibody (66039-1-Ig, Clone: 5D8G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

• 

  FC experiment of HeLa using 66039-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CPT1A Monoclonal antibody (66039-1-Ig, Clone:5D8G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

66039-1-Ig targets CPT1A in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CPT1A fusion protein Ag7745
• Full Name: carnitine palmitoyltransferase 1A (liver)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC000185
• Gene Symbol: CPT1A
• Gene ID (NCBI): 1374
• RRID: AB_11041710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Publications

Species	Application	Title
mouse	WB	Diabetes; CEPT1-Mediated Phospholipogenesis Regulates Endothelial Cell Function and Ischemia-Induced Angiogenesis Through PPARα.; Authors - Mohamed A Zayed ; KD Validated
mouse	WB	Phytother Res; Angelica sinensis polysaccharide ameliorates nonalcoholic fatty liver disease via restoring estrogen-related receptor α expression in liver; Authors - Li Luo
mouse	IF	Commun Biol; Cpt1a Drives primed-to-naïve pluripotency transition through lipid remodeling; Authors - Zhaoyi Ma
human,rat	WB	Front Pharmacol; Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism; Authors - Tian Li
human	WB	Cancer Metab; MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation.; Authors - Ting Zhang
human	WB,IHC	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mohammad (Verified Customer) (04-29-2019)

working well, Without any non-specific band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse hippocampus