CRACR2A Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66787-1-Ig (same clone as 66787-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66787-1-Ig (CRACR2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66787-1-Ig (same clone as 66787-1-PBS)

  WB result of CRACR2A antibody (66787-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRACR2A transfected Jurkat cells. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66787-1-Ig (same clone as 66787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66787-1-Ig (CRACR2A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66787-1-Ig (same clone as 66787-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66787-1-Ig (CRACR2A antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51547-1

  Cytometric bead array sample test of MP51547-1, EFCAB4B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66787-1-PBS. Detection antibody: 66787-2-PBS.

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  Cytometric bead array standard curve of MP51547-1

  Cytometric bead array standard curve of MP51547-1, EFCAB4B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66787-1-PBS. Detection antibody: 66787-2-PBS. Standard:Ag7525. Range: 0.391-50 ng/mL.

• Featured Product
• KD/KO Validated

CRACR2A Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 66787-1-PBS

Clone No.4C1F5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

EFCAB4B, 4C1F5, Calcium release-activated calcium channel regulator 2A, Calcium release-activated channel regulator 2A, CRAC channel regulator 2A

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Product Information

66787-1-PBS targets CRACR2A as part of a matched antibody pair:

MP51547-1: 66787-1-PBS capture and 66787-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7525

  Product name: Recombinant human EFCAB4B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-395 aa of BC004524

  Sequence: MAAPDGRVVSRPQRLGQGSGQGPKGSGACLHPLDSLEQKETQEQTSGQLVMLRKAQEFFQTCDAEGKGFIARKDMQRLHKELPLSLEELEDVFDALDADGNGYLTPQEFTTGFSHFFFSQNNPSQEDAGEQVAQRHEEKVYLSRGDEDLGDMGEDEEAQFRMLMDRLGAQKVLEDESDVKQLWLQLKKEEPHLLSNFEDFLTRIISQLQEAHEEKNELECALKRKIAAYDEEIQHLYEEMEQQIKSEKEQFLLKDTERFQARSQELEQKLLCKEQELEQLTQKQKRLEGQCTALHHDKHETKAENTKLKLTNQELARELERTSWELQDAQQQLESLQQEACKLHQEKEMEVYRVTESLQREKAGLLKQLDFLRCVGGHWPVLRAPPRSLGSEGPV

• Full Name: EF-hand calcium binding domain 4B
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC004524
• Gene Symbol: CRACR2A
• Gene ID (NCBI): 84766
• RRID: AB_2882132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9BSW2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.