CRBN Polyclonal antibody

• Featured Product
• KD/KO Validated

CRBN Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (6)

Cat no : 11435-1-AP

Synonyms

cereblon, CRBN, MRT2A, Protein cereblon

---

Validation Data Gallery

• 

  WB analysis of mouse heart using 11435-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11435-1-AP (CRBN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11435-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11435-1-AP (CRBN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11435-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11435-1-AP (CRBN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11435-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11435-1-AP (CRBN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11435-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11435-1-AP (CRBN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11435-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11435-1-AP (CRBN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11435-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11435-1-AP (CRBN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11435-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11435-1-AP (CRBN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 11435-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11435-1-AP (CRBN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cervical cancer using 11435-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11435-1-AP (CRBN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of COLO 320 using 11435-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed COLO 320 cells using 11435-1-AP (CRBN antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A375 using 11435-1-AP

  Immunofluorescent analysis of A375 cells using 11435-1-AP (CRBN antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, COLO 320 cells, human brain tissue, human heart tissue, A375 cells, mouse liver tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: COLO 320 cells, A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IP: See 2 publications below

Product Information

11435-1-AP targets CRBN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRBN fusion protein Ag1951
• Full Name: cereblon
• Calculated Molecular Weight: 442 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC017419
• Gene Symbol: CRBN
• Gene ID (NCBI): 51185
• RRID: AB_2085739
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).

Publications

Species	Application	Title
mouse	WB	Science; DRUG DEVELOPMENT. Phthalimide conjugation as a strategy for in vivo target protein degradation.; Authors - Georg E Winter ; KO Validated
human	WB	Mol Cell; BET Bromodomain Proteins Function as Master Transcription Elongation Factors Independent of CDK9 Recruitment.; Authors - Georg E Winter ; KO Validated
human	WB	Nat Commun; Homo-PROTACs: bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradation.; Authors - Chiara Maniaci
human	WB	Cell Rep; The Drug Vehicle and Solvent N-Methylpyrrolidone Is an Immunomodulator and Antimyeloma Compound.; Authors - Jake Shortt
human	WB	Elife; Targeted degradation of BRD9 reverses oncogenic gene expression in synovial sarcoma.; Authors - Gerard L Brien
mouse	WB	FASEB J; AMPK is down-regulated by the CRL4A-CRBN axis through the polyubiquitination of AMPKα isoforms.; Authors - Eunju Kwon ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Elisa (Verified Customer) (02-07-2022)

RPE1 cells were fixed in cold methanol for 10' at -20C . Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

CRBN Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:11435-1-AP)

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jing (Verified Customer) (01-27-2020)

Tested in human hiPSC-derived cardiomyocytes, it showed very clean single band around 60 kd.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cardiomyocytes

FH

Yan (Verified Customer) (01-03-2020)

We tested CBRN antibody in mouse heart and brain tissue extracts. CRBN antibody have specificity to recognize CRBN by western blot which was confirmed by knockout mice.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain and heart tissue

FH

Jie (Verified Customer) (12-27-2019)

works specifically with one clear band at the right size

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cardiomtocytes

FH

Sarah (Verified Customer) (07-03-2019)

Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-CRBN in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U251/Jurkat

CRBN Antibody Western Blot, validation (1:1000 dilution) in U251/Jurkat (Cat no:11435-1-AP)

Related Products

AMPK Alpha Polyclonal antibody

10929-2-AP

NF-κB p65 Polyclonal antibody

10745-1-AP

SOCS1 Polyclonal antibody

25852-1-AP