Tested Applications
| Positive WB detected in | C6 cells, 3T3-L1 cells, COLO 320 cells, mouse liver tissue, U-251 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | COLO 320 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28494-1-AP targets CRBN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29353 Product name: Recombinant human CRBN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC017419 Sequence: MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAK Predict reactive species |
| Full Name | cereblon |
| Calculated Molecular Weight | 442 aa, 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC017419 |
| Gene Symbol | CRBN |
| Gene ID (NCBI) | 51185 |
| RRID | AB_2881155 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96SW2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CRBN antibody 28494-1-AP | Download protocol |
| IHC protocol for CRBN antibody 28494-1-AP | Download protocol |
| WB protocol for CRBN antibody 28494-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CRBN Polyclonal antibody (catalog number 28494-1-AP) has 13 total citations. These appear in journals including Nature Communications, Acta Pharmaceutica Sinica B, Asian Journal of Pharmaceutical Sciences, Molecular Therapy, Cell Death Discovery, PNAS, Leukemia, British Journal of Pharmacology, Journal of Medicinal Chemistry, Investigative Ophthalmology & Visual Science, Bioorganic Chemistry, Human Molecular Genetics, and Cell Signaling. Research fields span PROTAC/targeted protein degradation, cancer therapy (leukemia, myeloma, hepatocellular carcinoma, breast cancer), neurodegenerative disease, renal fibrosis, and immunotherapy.
| Species | Application | Title |
|---|---|---|
Nat Commun DNA nanoflower Oligo-PROTAC for targeted degradation of FUS to treat neurodegenerative diseases | ||
Acta Pharm Sin B Discovery of a potent PROTAC degrader for RNA demethylase FTO as antileukemic therapy | ||
Cell Death Discov Arginine methylation-dependent TRIM47 stability mediated by CARM1 promotes the metastasis of hepatocellular carcinoma | ||
Proc Natl Acad Sci U S A Negative interplay between HIV-1 Gag and amyloid precursor protein centers around competition for VPS4A and TSG101 |
Reviews
What customers say
The antibody received a 5-star rating for Western Blot. At a 1:6000 dilution, it detected a band around 50 kD in HEK293, A549, H460, HeLa, and MCF7 cell lysates. The protocol involved a 1-hour room-temperature incubation, followed by a donkey anti-rabbit (Alexa Fluor 800) secondary antibody at 1:20000 dilution for 1 hour at room temperature. Overall, the reviewer reported clear and reliable detection performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Angie (Verified Customer) (07-06-2023) | The antibody detected a band around 50 kD at dilution of 1:6000 incubated at room temperature for 1 hour followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 1 hour at room temperature.
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