CREB3L2 Polyclonal antibody

• 

  WB analysis using 14514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14514-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 14514-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CREB3L2 antibody (14514-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

CREB3L2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14514-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

BBF2H7, cAMP-responsive element-binding protein 3-like protein 2, CREB3L 2, Cyclic AMP-responsive element-binding protein 3-like protein 2, Processed cyclic AMP-responsive element-binding protein 3-like protein 2

---

Tested Applications

• Positive WB detected in: Jurkat cells, mouse liver tissue, K-562 cells, SH-SY5Y cells, Neuro-2a cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

14514-1-AP targets CREB3L2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5973

  Product name: Recombinant human CREB3L2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-247 aa of BC063666

  Sequence: MEVLESGEQGVLQWDRKLSELSEPGDGEALMYHTHFSELLDEFSQNVLGQLLNDPFLSEKSVSMEVEPSPTSPAPLIQAEHSYSLCEEPRAQSPFTHITSDSFNDDEVESEKWYLSTDFPSTSIKTEPVTDEPPPGLVPSVTLTITAISTPLEKEEPPLEMNTGVDSSCQTIIPKIKLEPHEVDQFLNFSPKEGLSALPVSLWVMDMVSGSTEREYGERAGMSLYHRCCSWLYEIALFLKNKNFASK

• Full Name: cAMP responsive element binding protein 3-like 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC063666
• Gene Symbol: CREB3L2
• Gene ID (NCBI): 64764
• RRID: AB_2292123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q70SY1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclic AMP-responsive element-binding protein 3-like protein 2 (CREB3L2), also named BBF2H7, is a transcriptional activator which is activated during endoplasmic reticulum (ER) stress late phase. It has 3 isoforms produced by alternative splicing. CREB3L2 exerts protective effect in neurons by regulation of unfolded protein response (UPR)-dependent gene transcriptions. N-terminal cytoplamic domain of CREB3L2 is cleaved under ER stress and then translocates to the nucleus. Catalog#14514-1-AP is a rabbit polyclonal antibody raised against N-terminal (1-247aa) of human CREB3L2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Br J Pharmacol; Azithromycin alleviates the severity of rheumatoid arthritis by targeting the unfolded protein response component of glucose-regulated protein 78 (GRP78).; Authors - Yongli Zhang
human	WB	Int J Mol Sci; Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells.; Authors - Kentaro Oh-Hashi
human	WB,IF	J Invest Dermatol; BBF2H7-mediated Sec23A pathway is required for endoplasmic reticulum-to-Golgi trafficking in dermal fibroblasts to promote collagen synthesis.; Authors - Ishikura-Kinoshita Seiko S
human	WB	J Biol Chem; APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10.; Authors - Liu Chao C
human	WB	Mol Biol Rep; Expression analysis and functional characterization of thioredoxin domain-containing protein 11; Authors - Ryoichi Murase
human	WB	Cell Biochem Biophys; Molecular characterization of the ER stress-inducible factor CRELD2; Authors - Shohei Hinaga