CREB3L2 Polyclonal antibody

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• CREB3L2 Polyclonal antibody (14514-1-AP)

CREB3L2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14514-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

BBF2H7, cAMP-responsive element-binding protein 3-like protein 2, CREB3L 2, Cyclic AMP-responsive element-binding protein 3-like protein 2, Processed cyclic AMP-responsive element-binding protein 3-like protein 2

• 

  WB analysis using 14514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 14514-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14514-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CREB3L2 antibody (14514-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse liver tissue, K-562 cells, SH-SY5Y cells, Neuro-2a cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14514-1-AP targets CREB3L2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5973

  Product name: Recombinant human CREB3L2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-247 aa of BC063666

  Sequence: MEVLESGEQGVLQWDRKLSELSEPGDGEALMYHTHFSELLDEFSQNVLGQLLNDPFLSEKSVSMEVEPSPTSPAPLIQAEHSYSLCEEPRAQSPFTHITSDSFNDDEVESEKWYLSTDFPSTSIKTEPVTDEPPPGLVPSVTLTITAISTPLEKEEPPLEMNTGVDSSCQTIIPKIKLEPHEVDQFLNFSPKEGLSALPVSLWVMDMVSGSTEREYGERAGMSLYHRCCSWLYEIALFLKNKNFASK

• Full Name: cAMP responsive element binding protein 3-like 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC063666
• Gene Symbol: CREB3L2
• Gene ID (NCBI): 64764
• RRID: AB_2292123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q70SY1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclic AMP-responsive element-binding protein 3-like protein 2 (CREB3L2), also named BBF2H7, is a transcriptional activator which is activated during endoplasmic reticulum (ER) stress late phase. It has 3 isoforms produced by alternative splicing. CREB3L2 exerts protective effect in neurons by regulation of unfolded protein response (UPR)-dependent gene transcriptions. N-terminal cytoplamic domain of CREB3L2 is cleaved under ER stress and then translocates to the nucleus. Catalog#14514-1-AP is a rabbit polyclonal antibody raised against N-terminal (1-247aa) of human CREB3L2.

Publications

What published studies show

CREB3L2 Polyclonal antibody (Cat# 14514-1-AP) has 8 total citations. These appear in journals including British Journal of Pharmacology, Science Signaling, Journal of Investigative Dermatology, International Journal of Molecular Sciences, Journal of Biological Chemistry, Molecular Biology Reports, and Cell Biochemistry and Biophysics. Research fields span rheumatoid arthritis, unfolded protein response, cognitive function, ER-to-Golgi trafficking, microRNA-mediated translation, glioma cytotoxicity, and ER stress.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Br J Pharmacol; Azithromycin alleviates the severity of rheumatoid arthritis by targeting the unfolded protein response component of glucose-regulated protein 78 (GRP78).; Authors - Yongli Zhang
human	WB	J Invest Dermatol; BBF2H7-mediated Sec23A pathway is required for endoplasmic reticulum-to-Golgi trafficking in dermal fibroblasts to promote collagen synthesis.; Authors - Ishikura-Kinoshita Seiko S
mouse	WB	Sci Signal; High glucose impairs cognitive function through Creb3 O-GlcNAcylation and increased lactate production.; Authors - Jingxi Xu
human	WB	Int J Mol Sci; Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells.; Authors - Kentaro Oh-Hashi
human	WB	J Biol Chem; APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10.; Authors - Liu Chao C
human	WB	Mol Biol Rep; Expression analysis and functional characterization of thioredoxin domain-containing protein 11; Authors - Ryoichi Murase