CRELD2 Polyclonal antibody

CRELD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27017-1-AP

Synonyms

CRELD2

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Validation Data Gallery

• 

  WB analysis of human placenta using 27017-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27017-1-AP (CRELD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 27017-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27017-1-AP (CRELD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 27017-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 27017-1-AP (CRELD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 27017-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27017-1-AP (CRELD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 27017-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 27017-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 27017-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 27017-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human placenta tissue, SH-SY5Y cells, U2OS cells
• Positive IHC detected in: human placenta tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 27017-1-AP is CRELD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRELD2 fusion protein Ag25553
• Full Name: cysteine-rich with EGF-like domains 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 30-45 kDa
• GenBank Accession Number: BC050675
• Gene Symbol: CRELD2
• Gene ID (NCBI): 79174
• RRID: AB_2880723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Ther; Ectopic clotting factor VIII expression and misfolding in hepatocytes as a cause for hepatocellular carcinoma; Authors - Audrey Kapelanski-Lamoureux