CREST Monoclonal antibody, PBS Only (Capture)

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  WB analysis of NIH/3T3 using 60314-1-Ig (same clone as 60314-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60314-1-Ig (same clone as 60314-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60314-1-Ig (same clone as 60314-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60314-1-Ig (same clone as 60314-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 60314-1-Ig (same clone as 60314-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51462-1

  Cytometric bead array sample test of MP51462-1, CREST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60314-1-PBS. Detection antibody: 60314-2-PBS.

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  Cytometric bead array standard curve of MP51462-1

  Cytometric bead array standard curve of MP51462-1, CREST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60314-1-PBS. Detection antibody: 60314-2-PBS. Standard:Ag3119. Range: 0.781-100 ng/mL.

CREST Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 60314-1-PBS

Clone No.3D5D10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

SS18L1, 3D5D10, Calcium-responsive transactivator, KIAA0693, LP2261

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Product Information

60314-1-PBS targets CREST as part of a matched antibody pair:

MP51462-1: 60314-1-PBS capture and 60314-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3119

  Product name: Recombinant human CREST protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 47-396 aa of BC034494

  Sequence: CTQYQQILHRNLVYLATIADSNQNMQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQGSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTLPTTSMSISGPGYSHAGPASQGVPMQGQGTIGNYVSRTNINMQSNPVSMIQQQAATSHYSSAQGGSQHYQGQSSIAMMGQGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGHGDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPSQQSYPGQQQGYGSAQGAPSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYGNYQQ

• Full Name: synovial sarcoma translocation gene on chromosome 18-like 1
• Calculated Molecular Weight: 396 aa, 43 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC034494
• Gene Symbol: CREST
• Gene ID (NCBI): 26039
• RRID: AB_2881425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75177
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).