CRHR1 Polyclonal antibody

CRHR1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 20967-1-AP

Synonyms

CRF R, CRF R 1, CRF R1, CRF1, CRF1R, CRFR, CRFR 1, CRFR1, CRH R 1, CRH R1, CRH R1h, CRHR, CRHR1, CRHR1f



Tested Applications

Positive WB detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

20967-1-AP targets CRHR1 in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name corticotropin releasing hormone receptor 1
Calculated molecular weight 48 kDa
Observed molecular weight 46 kDa
GenBank accession numberNM_004382
Gene symbol CRHR1
Gene ID (NCBI) 1394
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Corticotropin-releasing hormone (CRH, also known as Corticotropin-releasing factor, CRF) is a 41 amino acid peptide hormone and a key regulator of the stress response. CRH and the related urocortin peptides (UCN, UCN2 and UCN3) mediate their actions through two mammalian receptor subtypes, CRHR1 and CRHR2 (PMID: 23863939). CRHR1 (also known as CRFR1) exhibits high affinity towards CRH and UCN but low affinity towards UCN2 and no affinity towards UCN3 (PMID: 22684850). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Widely distributed through out the brain, CRHR1 critically controls behavioral adaptation to stress and is causally linked to emotional disorders (PMID: 21885734). Alternative splicing results in multiple transcript variants encoding distinct isoforms of CRHR1. This antibody raised against a synthesized peptide corresponding to amino acid residues 148-165 of isoform CRF-R1 can only recognize isoform CRF-R1.

Protocols

Product Specific Protocols
WB protocol for CRHR1 antibody 20967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Biochim Biophys Sin (Shanghai)

Effects of baicalein on pancreatic cancer stem cells via modulation of sonic Hedgehog pathway.

Authors - Libin Song
WB

Reprod Toxicol

Dynamic expression of corticotropin-releasing hormone and urocortin in estrogen induced-cholestasis pregnant rat.

Authors - Fan Zhou
ratWB

Brain Res

Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Authors - Di Wang
ratWB

Int J Mol Med

Sini San ameliorates duodenal mucosal barrier injury and low‑grade inflammation via the CRF pathway in a rat model of functional dyspepsia.

Authors - Chunyang Zhu
ratWB

Behav Brain Res

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Authors - Shuai Lian
IF

Proc Natl Acad Sci U S A

Overactivation of corticotropin-releasing factor receptor type 1 and aquaporin-4 by hypoxia induces cerebral edema.

Authors - Shao-Jun Chen