CRIPT Polyclonal antibody

• Featured Product
• KD/KO Validated

CRIPT Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11211-1-AP

Synonyms

HSPC139, Cysteine-rich PDZ-binding protein, Cysteine-rich interactor of PDZ3, Cysteine-rich interactor of PDZ three

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Validation Data Gallery

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  WB analysis of L02 using 11211-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11211-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11211-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11211-1-AP

  IP result of anti-CRIPT (IP:11211-1-AP, 4ug; Detection:11211-1-AP 1:500) with L02 cells lysate 1800ug.

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  IF Staining of L02 using 11211-1-AP

  Immunofluorescent analysis of L02 cells using 11211-1-AP (CRIPT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IP detected in: L02 cells
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

11211-1-AP targets CRIPT in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRIPT fusion protein Ag1708
• Full Name: cysteine-rich PDZ-binding protein
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC018653
• Gene Symbol: CRIPT
• Gene ID (NCBI): 9419
• RRID: AB_2084731
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The clustering and immobilization of receptors and ion channels located at specific subcellular sites are believed to be mediated by intracellular proteins that attach these membrane proteins to the cytoskeleton. Receptors and ion channels interact with cytoplasmic proteins, which involved in the modulation or downstream signaling mechanisms of the receptor/ion channel. CRIPT is the protein that binds to the third PDZ (PDZ3) domain of PSD95, which binds to and clusters a variety of membrane proteins, including Shaker-type potassium channel subunits and NMDA receptor NR2 subunits, via its first 2 N-terminal PDZ domains

Publications

Species	Application	Title
human	IF	Nat Commun; m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation; Authors - Pierre Klein
rat	WB,CoIP	Mol Neurobiol; Interaction Between CRIPT and PSD-95 Is Required for Proper Dendritic Arborization in Hippocampal Neurons.; Authors - Anton Omelchenko ; KD Validated
rat	WB,IP	eNeuro; SAP97 Binding Partner CRIPT Promotes Dendrite Growth In Vitro and In Vivo.; Authors - Lei Zhang