CRISP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CRISP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 19066-1-AP

Synonyms

Cancer/testis antigen 36, CRISP 2, CRISP2, CT36, GAPDL5, Testis specific protein TPX 1, TPX1, TSP1

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Validation Data Gallery

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  WB analysis using 19066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19066-1-AP (CRISP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19066-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19066-1-AP (CRISP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19066-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19066-1-AP (CRISP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19066-1-AP

  IP result of anti-CRISP2 (IP:19066-1-AP, 4ug; Detection:19066-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 19066-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19066-1-AP (CRISP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 19066-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19066-1-AP (CRISP2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below

Product Information

The immunogen of 19066-1-AP is CRISP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRISP2 fusion protein Ag13574
• Full Name: cysteine-rich secretory protein 2
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC022011
• Gene Symbol: CRISP2
• Gene ID (NCBI): 7180
• RRID: AB_10667008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nat Commun; PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.; Authors - Deqiang Ding
mouse	WB	Nucleic Acids Res; DAZAP1 regulates the splicing of Crem, Crisp2 and Pot1a transcripts.; Authors - Chen Hsiang-Ying HY
mouse	WB	FASEB J; Functional redundancy and compensation: Deletion of multiple murine Crisp genes reveals their essential role for male fertility.; Authors - L Curci
human	WB	Biol Reprod; The Expression of Cysteine-Rich Secretory Protein 2 (CRISP2) and Its Specific Regulator miR-27b in the Spermatozoa of Patients with Asthenozoospermia.; Authors - Jun-Hao Zhou
mouse	WB	Mol Hum Reprod; Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders.; Authors - N G Brukman ; KO Validated
pig	IF,WB	Biol Reprod; Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa.; Authors - M Zhang