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  • KD/KO Validated

CRISP2 Polyclonal antibody

CRISP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19066-1-AP

Synonyms

Cancer/testis antigen 36, CRISP 2, CRISP2, CT36, GAPDL5, Testis specific protein TPX 1, TPX1, TSP1



Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19066-1-AP is CRISP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRISP2 fusion protein Ag13574
Full Name cysteine-rich secretory protein 2
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC022011
Gene Symbol CRISP2
Gene ID (NCBI) 7180
RRIDAB_10667008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CRISP2 antibody 19066-1-APDownload protocol
IHC protocol for CRISP2 antibody 19066-1-APDownload protocol
IP protocol for CRISP2 antibody 19066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.

Authors - Deqiang Ding
mouseWB

Nucleic Acids Res

DAZAP1 regulates the splicing of Crem, Crisp2 and Pot1a transcripts.

Authors - Chen Hsiang-Ying HY
mouseWB

FASEB J

Functional redundancy and compensation: Deletion of multiple murine Crisp genes reveals their essential role for male fertility.

Authors - L Curci
humanWB

Biol Reprod

The Expression of Cysteine-Rich Secretory Protein 2 (CRISP2) and Its Specific Regulator miR-27b in the Spermatozoa of Patients with Asthenozoospermia.

Authors - Jun-Hao Zhou
mouseWB

Mol Hum Reprod

Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders.

Authors - N G Brukman
  • KO Validated
pigIF,WB

Biol Reprod

Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa.

Authors - M Zhang