CRLS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CRLS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 51055-1-AP

Synonyms

C20orf155, Cardiolipin synthase, cardiolipin synthase 1, CLS, CLS1, CRLS1, GCD10, Protein GCD10 homolog

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Validation Data Gallery

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  WB analysis of mouse heart using 51055-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 51055-1-AP (CRLS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51055-1-AP (CRLS1 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue, human breast cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

51055-1-AP targets CRLS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cardiolipin synthase 1
• Calculated Molecular Weight: 301 aa, 32 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC069010
• Gene Symbol: CRLS1
• Gene ID (NCBI): 54675
• RRID: AB_2085334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiolipin (CL) is a mitochondrial specific double negatively charged phospholipid which is intricately involved in regulating bioenergetic efficiency. In eukaryotes, CL is synthesized by cardiolipin synthase (CRLS1 or CLS1) which catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form CL and glycerol. Recently it has been reported that transgenic expression of CRLS1 accelerates cardiolipin remodeling, improves mitochondrial function, modulates mitochondrial signaling, and attenuates mitochondrial dysfunction during diabetes, thereby identifying CRLS1 as a novel therapeutic target to attenuate mitochondrial dysfunction in diabetic myocardium. The predicted molecular weight of CRLS1 is around 32-35 kDa, while a 50 kDa protein with CRLS1 activity had been observed in liver isolation, which may represent an isoform of CRLS1 (20652826).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice; Authors - Hongming Su
mouse	WB	Hepatology; Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury; Authors - Li Chen ; KD Validated