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Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody, PBS Only (98157-1-PBS)

Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98157-1-PBS-1MG

CRTAM/CD355 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98157-1-PBS

Clone No. 241280B12

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

CRTAM, 241280B12, CD355, Class-I MHC-restricted T-cell-associated molecule, Cytotoxic and regulatory T-cell molecule

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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FC experiment of human PBMCs using 98157-1-RR (same clone as 98157-1-PBS)
1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD8. Cells were not fixed. This data was developed using the same antibody clone with 98157-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD8. Cells were not fixed. This data was developed using the same antibody clone with 98157-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 98157-1-RR (same clone as 98157-1-PBS)
1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG (dashed, black). Cells were not fixed. This data was developed using the same antibody clone with 98157-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG (dashed, black). Cells were not fixed. This data was developed using the same antibody clone with 98157-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98157-1-RR
Biolayer interferometry (BLl) kinetic assays of 98157-1-RR against Human CRTAM/CD355 were performed. The affinity constant is 0.449 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

98157-1-PBS targets CRTAM/CD355 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1541 Product name: Recombinant Human CRTAM protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 18-286 aa of NM_019604.4 Sequence: SLTNHTETITVEEGQTLTLKCVTSLRKNSSLQWLTPSGFTIFLNEYPALKNSKYQLLHHSANQLSITVPNVTLQDEGVYKCLHYSDSVSTKEVKVIVLATPFKPILEASVIRKQNGEEHVVLMCSTMRSKPPPQITWLLGNSMEVSGGTLHEFETDGKKCNTTSTLIIHTYGKNSTVDCIIRHRGLQGRKLVAPFRFEDLVTDEETASDALERNSLSSQDPQQPTSTVSVTEDSSTSEIDKEEKEQTTQDPDLTTEANPQYLGLARKKS Predict reactive species
Full Name	cytotoxic and regulatory T cell molecule
Calculated Molecular Weight	45 kDa
GenBank Accession Number	NM_019604.4
Gene Symbol	CRTAM
Gene ID (NCBI)	56253
RRID	AB_3672300
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O95727-1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223).