Anti-Mouse CRTAM/CD355 Rabbit Recombinant Antibody

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• Anti-Mouse CRTAM/CD355 Rabbit Recombinant Antibody (98420-3-RR)

CRTAM/CD355 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98420-3-RR Copied!

Clone No. 250055A1

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD355, Class-I MHC-restricted T-cell-associated molecule, Crtam, Cytotoxic and regulatory T-cell molecule

• 

  FC experiment of mouse splenocytes using 98420-3-RR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CRTAM/CD355 Rabbit RecAb (98420-3-RR, Clone: 250055A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD8a (53-6.7) (APC-65069, Clone: 53-6.7). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of mouse splenocytes using 98420-3-RR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CRTAM/CD355 Rabbit RecAb (98420-3-RR, Clone: 250055A1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse Crtam(CD355 ) Rabbit RecAb (98420-3-RR, Clone:250055A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98420-3-RR targets CRTAM/CD355 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2691

  Product name: Recombinant Mouse CRTAM/CD355 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 17-284 aa of NM_001281954.1

  Sequence: AFLKMETVTVEEGQTLTLTCVTSQTKNVSLQWLAPSGFTIFLNQHPALKSSKYQLLHHSATQLSISVSNVTLREEGVYTCLHYGSSVKTKQVRVTVLVTPFQPTVEALVLRRQNGEKSVVLKCSTERSKPPPQITWLLGEGLEIYGELNHEFEADGKICNTSSMLIARAYGKNSTVHCIIQHEGLHGRKLVAPFQFEDLVADQETSDQETSDAPEQSSLSSQALQQPTSTVSMMENSSIPETDKEEKEHATQDPGLSTEASAQHTGLA

• Full Name: cytotoxic and regulatory T cell molecule
• Calculated Molecular Weight: 44 kDa
• GenBank Accession Number: NM_001281954.1
• Gene Symbol: Crtam
• Gene ID (NCBI): 54698
• RRID: AB_3746061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q149L7-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223).